Abstract
A Gram-positive, motile, rod-shaped and lignin-degrading novel actinomycete, designated strain NEAU-YY56T, was isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, Central China and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-YY56T belonged to the genus Cellulomonas and exhibited 16S rRNA gene sequence similarities of 98.7, 98.2 and 98.1% to Cellulomonas pakistanensis JCM 18755T, Cellulomonas denverensis JCM 14733T and Cellulomonas hominis JCM 12133T, respectively. The whole-cell sugars were glucose, rhamnose and ribose. The peptidoglycan of strain NEAU-YY56T contained ornithine and glutamic acid. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and two unknown glycolipids. The major menaquinone was MK-9(H4). The major fatty acids (> 5.0%) were identified as anteiso-C15:0, C16:0, C14:0 and anteiso-C17:0. Meanwhile, DNA G+C content was 74.7%. The morphological and chemotaxonomic properties of strain NEAU-YY56T were also confirmed the affiliation of the isolate to the genus Cellulomonas. However, physiological and biochemical characteristics indicated that strain NEAU-YY56T can be clearly differentiated from its closest relatives. In addition, the ANI values and dDDH levels between strain NEAU-YY56T and related Cellulomonas species were lower than the accepted threshold value. Therefore, it is concluded that strain NEAU-YY56T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas triticagri sp. nov. is proposed. The type strain is NEAU-YY56T (= DSM 106717T = JCM 32550T).
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Abbreviations
- Ala:
-
Alanine
- ANI:
-
Average nucleotide identity
- Asp:
-
Aspartic acid
- atpD:
-
ATP synthase F1, beta subunit
- dDDH:
-
Digital DNA:DNA hybridization
- DPA:
-
Dulcitol-proline agar
- DPG:
-
Diphosphatidylglycerol
- DSM:
-
Deutsche Sammlung von Mikroorganismen und Zellkulturen
- GC–MS:
-
Gas Chromatography–Mass Spectrometry
- GL:
-
Unknown glycolipid
- Glu:
-
Glutamic acid
- GO:
-
Gene Ontology
- gyrB:
-
DNA gyrase subunit B
- ISCC-NBS:
-
Inter-Society Color Council-National Bureau of Standards
- ISP:
-
International Streptomyces Project
- ISP 2:
-
Yeast extract–malt extract agar
- ISP 3:
-
Oatmeal agar
- ISP 4:
-
Inorganic salt-starch agar
- ISP 5:
-
Glycerol-asparagine agar
- ISP 6:
-
Peptone-yeast extract iron agar
- ISP 7:
-
Tyrosine agar
- JCM:
-
Japan Collection of Microorganisms
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- MEGA:
-
Molecular Evolutionary Genetics Analysis
- MLSA:
-
Multilocus sequence analysis
- Orn:
-
Ornithine
- PE:
-
Phosphatidylethanolamine
- PGAP:
-
Prokaryotic Genome Annotation Pipeline
- PIM:
-
Phosphatidylinositol mannoside
- recA:
-
Recombinase A
- relA:
-
PpGpp synthetase
- rpoB:
-
RNA polymerase, beta subunit
- TSA:
-
Trypticase soy agar
- TSB:
-
Trypticase soy both
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Acknowledgements
This work was supported in part by grants from the Youth Scientific Research Fund of Liaoning University (LDQN-202010), People's Livelihood Science and Technology Program of Liaoning Provincial Department of Science and Technology (2021JH2/10200015), and General Research Project Found of Liaoning Province Education Department (LJC201910). We are grateful to Professor Aharon Oren (The Hebrew University of Jerusalem) for helpful advice on the epithet.
Funding
This work was funded by Youth Scientific Research Fund of Liaoning University (Grant No. LDQN-202010), People's Livelihood Science and Technology Program of Liaoning Provincial Department of Science and Technology (Grant No. 2021JH2/10200015), General Research Project Found of Liaoning Province Education Department (Grant no. LJC201910).
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CH and YZ performed the laboratory experiments, analyzed the data, and drafted the manuscript. BY contributed to the biochemical characterization. QS and YT contributed to the morphological analyzes. HS contributed to the fatty acids determination. YZ contributed to the Genomic analysis. JZ participated to the discussions of each section of experiments. WX and CZ designed the experiments and revised the manuscript.
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Communicated by Erko Stackebrandt.
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Han, C., Zhang, Y., Yu, B. et al. Cellulomonas triticagri sp. nov., isolated from the rhizosphere soil of wheat (Triticum aestivum L.). Arch Microbiol 204, 449 (2022). https://doi.org/10.1007/s00203-022-03036-z
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DOI: https://doi.org/10.1007/s00203-022-03036-z