Abstract
A novel Gram-stain-negative, aerobic, and rod-shaped bacterium with gliding motility, named strain ANRC-HE7T, was isolated from the seawater of Biological Bay adjacent to Fildes Peninsula, Antarctica. The optimal growth of this strain occurred at 28 °C, pH 7.5, and in the presence of 1.0% (w/v) NaCl. Strain ANRC-HE7T can produce amylase and harbors gene clusters involved in cellulose degradation. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain ANRC-HE7T formed a distinct lineage within the genus Maribacter and was closely related to Maribacter luteus RZ05T (98.4% sequence similarity), Maribacter polysiphoniae LMG 23671T (98.3%), and Maribacter arenosus CAU 1321T (97.3%). However, digital DNA–DNA hybridization and average nucleotide identity values between strain ANRC-HE7T and closely related strains were 17.4–49.1% and 70.9–92.7%, much lower than the cutoff values of 70% and 95%, respectively. On the other hand, strain ANRC-HE7T shared characteristics with most type strains within the genus. Its respiratory quinone was MK-6. The major fatty acids were iso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and anteiso-C15:0. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids, four unidentified phospholipids, and five unidentified glycolipids. The DNA G + C content of strain ANRC-HE7T was 40.1%. Based on the results of the biochemical, phylogenetic, and chemotaxonomic analyses, strain ANRC-HE7T is suggested to represent a novel species of the genus Maribacter, for which the name Maribacter aquimaris sp. nov. is proposed. The type strain is ANRC-HE7T (= MCCC 1K03787T = KCTC 72532T).
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The GenBank accession numbers of the 16S rRNA gene and the draft genome sequences of strain ANRC-HE7T are MN658399 and JABTCF000000000, respectively. All other data generated or analysed during this study are included in this published article and its supplementary information files.
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Acknowledgements
Funding was provided by Special Foundation for National Science and Technology Basic Research Program of China (No. 2019FY100706), the Science Fund for Distinguished Young Scholars of Anhui Province (No. 2008085J12), and the Science and Technology Major Project of Anhui Province (No. 202103a06020006).
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YFZ, YWZ, LLM, WF, and MMH performed the experiments and wrote the manuscript, YZX and ZMF designed the study and revised the manuscript. All the authors read and approved the final version of the manuscript.
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Zhang, Y., Zhai, Y., Mu, L. et al. Maribacter aquimaris sp. nov., isolated from seawater adjacent to Fildes Peninsula, Antarctica. Antonie van Leeuwenhoek 116, 753–761 (2023). https://doi.org/10.1007/s10482-023-01844-x
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DOI: https://doi.org/10.1007/s10482-023-01844-x