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Marivivens aquimaris sp. nov., isolated from seawater

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Abstract

A Gram-stain-negative, strictly aerobic, non-flagellated, rod-shaped bacterium, designated GSB7T, was isolated from seawater collected at the Yellow Sea coast of South Korea. Catalase and oxidase activities were positive. Growth occurred at pH 6.0–9.0 (optimum pH 7.0), 10–40 °C (optimum 30 °C) and with 0–8% NaCl (optimum 1–2%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSB7T belonged to the genus Marivivens, showing the sequence similarities of 96.3, 96.1, and 96.0% with Marivivens niveibacter HSLHS2T, Limimaricola hongkongensis DSM17492T, and Marivivens donghaensis AM-4T, respectively. The respiratory quinone was ubiquinone-10 and the major fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C18:1 ω7c 11-methyl, C16:0 and C10:0 3-OH. The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid, and five unidentified lipids. The DNA G + C content calculated from the whole-genome sequence was 60.6 mol%. On the basis of phenotypic, chemotaxonomic and genotypic characteristics presented in this study, strain GSB7T is suggested to represent a novel species of the genus Marivivens, for which the name Marivivens aquimaris sp. nov. is proposed. The type strain is GSB7T (= KCTC 82026T = JCM 34042T).

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Abbreviations

MA:

Marine agar

MB:

Marine broth

KCTC:

Korean Collection for Type Cultures

JCM:

Japan Collection of Microorganisms

NJ:

Neighbor-joining

ML:

Maximum-likelihood

MP:

Maximum-parsimony

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

UBCG:

Up-to-date bacterial core gene set

NA:

Nutrient agar

TSA:

Tryptic soy agar

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Acknowledgements

This study was supported by the Technology Development Program to solve climate changes (Systems Metabolic Engineering for Biorefineries) from the Ministry of Science and ICT through the National Research Foundation (NRF) of Korea and a basic research grant from the Korea Research Institute of Bioscience and Biotechnology (KRIBB).

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Author notes

  1. Jeong Ah Kim and Binna Lee equally contributed to this work.

Authors and Affiliations

  1. Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, Jeonbuk, 56212, Republic of Korea

    Jeong Ah Kim, Binna Lee, Ji Young Kang, Jae Jun Song & Jong Hyun Choi

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  1. Jeong Ah Kim
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  2. Binna Lee
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  3. Ji Young Kang
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Correspondence to Jae Jun Song or Jong Hyun Choi.

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Communicated by Erko Stackebrandt.

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Kim, J.A., Lee, B., Kang, J.Y. et al. Marivivens aquimaris sp. nov., isolated from seawater. Arch Microbiol 203, 3229–3234 (2021). https://doi.org/10.1007/s00203-021-02305-7

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