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Burkholderia metalliresistens sp. nov., a multiple metal-resistant and phosphate-solubilising species isolated from heavy metal-polluted soil in Southeast China

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Abstract

A metal-resistant and phosphate-solubilising bacterium, designated as strain D414T, was isolated from heavy metal (Pb, Cd, Cu and Zn)-polluted paddy soils at the surrounding area of Dabao Mountain Mine in Southeast China. The minimum inhibitory concentrations of heavy metals for strain D414T were 2000 mg L−1 (Cd), 800 mg L−1 (Pb), 150 mg L−1 (Cu) and 2500 mg L−1 (Zn). The strain possessed plant growth-promoting properties, such as 1-aminocyclopropane-1-carboxylate assimilation, indole production and phosphate solubilisation. Analysis of 16S rRNA gene sequence indicated that the isolate is a member of the genus Burkholderia where strain D414T formed a distinct phyletic line with validly described Burkholderia species. Strain D414T is closely related to Burkholderia tropica DSM 15359T, B. bannensis NBRC E25T and B. unamae DSM 17197T, with 98.5, 98.3 and 98.3 % sequence similarities, respectively. Furthermore, less than 34 % DNA–DNA relatedness was detected between strain D414T and the type strains of the phylogenetically closest species of Burkholderia. The dominant fatty acids of strain D414T were C14:0, C16:0, C17:0 cyclo and C18:1 ω7c. The DNA G+C content was 62.3 ± 0.5 mol%. On the basis of genotypic, phenotypic and phylogenetic data, strain D414T represents a novel species, for which the name Burkholderia metalliresistens sp. nov. is proposed, with D414T (=CICC 10561T = DSM 26823T) as the type strain

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Abbreviations

MIC:

Minimum inhibitory concentration

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Acknowledgments

We wish to thank J.P. Euzéby for the helpful advice on etymology of bacterial names. We also acknowledge JinXia Li at the CICC and Dr. Sabine Gronow at the DSMZ for deposit of the isolates. This research was financially supported by the Central Public Research Institutes Basic Funds for Research and Development (Agro-Environmental Protection Institute, Ministry of Agriculture) and National Natural Science Foundation of China (41473115).

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Authors and Affiliations

  1. Innovation Team of Remediation for Heavy Metal Contaminated Farmland of Chinese Academic of Agricultural Sciences, Institute of Agro-Environmental Protection, Ministry of Agriculture, Tianjin, 300191, People’s Republic of China

    Jun Kang Guo, Ren Wei Feng, Rui Gang Wang & Ying Ming Xu

  2. Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin, 300191, People’s Republic of China

    Yong Zhen Ding

  3. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Xiu Li Wei & Wei Min Chen

  4. Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, 40506, USA

    Chun Chen

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  1. Jun Kang Guo
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Correspondence to Jun Kang Guo or Wei Min Chen.

Additional information

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strain D414T is KF601211.

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Guo, J.K., Ding, Y.Z., Feng, R.W. et al. Burkholderia metalliresistens sp. nov., a multiple metal-resistant and phosphate-solubilising species isolated from heavy metal-polluted soil in Southeast China. Antonie van Leeuwenhoek 107, 1591–1598 (2015). https://doi.org/10.1007/s10482-015-0453-z

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