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Effect of Xylan and Lignin Removal by Hydrothermal Pretreatment on Enzymatic Conversion of Sugarcane Bagasse Cellulose for Second Generation Ethanol Production

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Abstract

This work was aimed to evaluate the effect of the removal of hemicellulose and lignin, by hydrothermal pretreatment, carried out at four different temperatures, namely 180, 185, 190 and 195 °C, for 10 min in a 20-L reactor, and alkaline delignification with 1.0 % (w/v) NaOH, at 100 °C for 1 h, on the enzymatic saccharification of sugarcane bagasse cellulose. For the material pretreated under the most severe conditions (1.0 % (w/v) NaOH, 100 °C, 1 h and 195 °C, 10 min), 95.8 % of the hemicellulosic fraction and 80.9 % of lignin were solubilised upon pretreatment and delignification respectively. The enzymatic conversion of the material obtained under those conditions reached 89.2 % of the initial cellulose, whereas it was 69.2 % for the pretreated but non-delignified material and only 6.0 % for raw bagasse. Models describing the effect of hemicellulose and lignin content on the enzymatic hydrolysis were developed. The statistical analysis of the results emphasized the significance of removal of the hemicellulose and lignin for improving the enzymatic hydrolysis of cellulose.

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Acknowledgments

The work was financially supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). Novozymes Latin America Ltd. is thanked for supplying the enzyme preparations. CM gratefully acknowledges the financial support given by the Alexander von Humboldt Foundation.

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Authors and Affiliations

  1. Laboratório Nacional de Ciência e Tecnologia do Bioetanol—CTBE, P.O. Box 6170, Campinas, São Paulo, CEP 13083-970, Brazil

    George J. M. Rocha, Vinícius F. N. Silva & Viviane Marcos Nascimento

  2. Department of Chemistry and Chemical Engineering, University of Matanzas, 44740, Matanzas, Cuba

    Carlos Martín

  3. vTI-Institute for Wood Technology and Wood Biology, Hamburg, 21031, Germany

    Carlos Martín

  4. Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, P.O. Box 116, Lorena, São Paulo, CEP 12602-810, Brazil

    Vinícius F. N. Silva & Adilson R. Gonçalves

  5. Departamento de Antibióticos, Universidade Federal de Pernambuco, P.O. Box 3447, Recife, Pernambuco, CEP 50100-100, Brazil

    Ana M. Souto-Maior

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  1. George J. M. Rocha
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  2. Vinícius F. N. Silva
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  3. Carlos Martín
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  4. Adilson R. Gonçalves
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  5. Viviane Marcos Nascimento
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  6. Ana M. Souto-Maior
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Correspondence to George J. M. Rocha.

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Rocha, G.J.M., Silva, V.F.N., Martín, C. et al. Effect of Xylan and Lignin Removal by Hydrothermal Pretreatment on Enzymatic Conversion of Sugarcane Bagasse Cellulose for Second Generation Ethanol Production. Sugar Tech 15, 390–398 (2013). https://doi.org/10.1007/s12355-013-0218-9

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