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Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells

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Abstract

The potential use of fed-batch cultivation (FBC) for improvement of the production of Lactobacillus salivarius I 24 biomass for subsequent use as probiotics was studied using a 2-L stirredtank bioreactor. Three different constant feeding rates (0.1, 0.05, and 0.033 L/h) were applied in FBCs and their effect on carbon metabolism was evaluated. The carbon flux for cell built-up with reduction in lactic acid synthesis was observed in the fed-batch as compared to the batch cultivation mode. The viable cell number obtained in the constant FBC (CFBC) operated at a feeding rate of 0.05 L/h was 8 times higher (10.7×1010 CFU/mL) than that recorded in the batch cultivation. This gave the viable cell yield based on glucose consumed for CFBC of 26 times higher (11.3×1012 CFU/gGlucose) than the batch cultivation. This study demonstrated CFBC, which is simple with minimal use of process control equipment, has an industrial potential for improvement of probiotic production.

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Authors and Affiliations

  1. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Lim Chi Ming, Murni Halim & Arbakariya Bin Ariff

  2. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Raha Abd Rahim

  3. Bioprocessing and Biomanufacturing Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Murni Halim & Arbakariya Bin Ariff

  4. Institute of Bioscience, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Ho Yin Wan

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  1. Lim Chi Ming
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  2. Murni Halim
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Correspondence to Murni Halim.

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Ming, L.C., Halim, M., Rahim, R.A. et al. Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells. Food Sci Biotechnol 25, 1393–1398 (2016). https://doi.org/10.1007/s10068-016-0217-1

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  • DOI: https://doi.org/10.1007/s10068-016-0217-1

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