Abstract
Hypersaline ecosystems host a particular microbiota, which can be specifically recruited by halophytes. In order to broaden our knowledge of hypersaline ecosystems, an in natura study was conducted on the microbiota associated with the halophyte Halocnemum strobilaceum from alkaline-saline arid soil in Algeria. We collected and identified a total of 414 strains isolated from root tissues (RT), root-adhering soil (RAS), non-adhering rhizospheric soil (NARS) and bulk soil (BS) using different NaCl concentrations. Our data showed that halophilic and halotolerant bacterial isolates in BS and the rhizosphere belonged to 32 genera distributed in Proteobacteria (49%), Firmicutes (36%), Actinobacteria (14%) and Bacteroidetes (1%). Bacterial population size and species diversity were greatly increased in the rhizosphere (factor 100). The reservoir of diversity in BS was dominated by the genera Bacillus and Halomonas. Bacillus/Halomonas ratio decreased with the proximity to the roots from 2.2 in BS to 0.3 at the root surface. Salt screening of the strains showed that species belonging to nine genera were able to grow up to 5.1 M NaCl. Thus, we found that H. strobilaceum exerted a strong effect on the diversity of the recruited microbiota with an affinity strongly attributed to the genus Halomonas.
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Data availability
Sequences of the strains used in this study were deposited at the National Center for Biotechnology Information Sequence Read Archive under the accession number PRJNA765921.
Abbreviations
- RT:
-
Root tissues
- RAS:
-
Root adhering soil
- NARS:
-
Non-adhering rhizospheric soil
- BS:
-
Bulk soil
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The authors thank Institut Technique de Développement de l’Agronomie Saharienne (ITDAS) for performing the pedological analysis.
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This work was supported by the French-Algerian Hubert Curien Partnership Program (PHC) TASSILI (14MDU907).
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SB, NB, TH and YK: designed the research. SB: carried out sample processing. WA, TH and YK: provided the experimental materials. SB: performed the laboratory work with contribution from NB. MB and PO: conducted bioinformatic data analysis. SB: wrote the first draft. TH and YK: supervised this study. All authors read, reviewed and edited previous versions of the manuscript. All authors approved the submission.
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Behairi, S., Baha, N., Barakat, M. et al. Bacterial diversity and community structure in the rhizosphere of the halophyte Halocnemum strobilaceum in an Algerian arid saline soil. Extremophiles 26, 18 (2022). https://doi.org/10.1007/s00792-022-01268-x
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DOI: https://doi.org/10.1007/s00792-022-01268-x