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The mechanism of lncRNA MALAT1 targeting the miR-124-3p/IGF2BP1 axis to regulate osteogenic differentiation of periodontal ligament stem cells

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Abstract

Objectives

This study aimed to investigate the regulatory roles of lncRNA MALAT1, miR-124-3p, and IGF2BP1 in osteogenic differentiation of periodontal ligament stem cells (PDLSCs).

Materials and methods

We characterized PDLSCs by employing quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analyses to evaluate the expression of key osteogenic markers including ALPL, SPP1, and RUNX2. Manipulation of lncRNA MALAT1 and miR-124-3p expression levels was achieved through transfection techniques. In addition, early osteogenic differentiation was assessed via Alkaline phosphatase (ALP) staining, and mineral deposition was quantified using Alizarin Red S (ARS) staining. Cellular localization of lncRNA MALAT1 was determined through Fluorescence In Situ Hybridization (FISH). To elucidate the intricate regulatory network, we conducted dual-luciferase reporter assays to decipher the binding interactions between lncRNA MALAT1 and miR-124-3P as well as between miR-124-3P and IGF2BP1.

Results

Overexpression of lncRNA MALAT1 robustly promoted osteogenesis in PDLSCs, while its knockdown significantly inhibited the process. We confirmed the direct interaction between miR-124-3p and lncRNA MALAT1, underscoring its role in impeding osteogenic differentiation. Notably, IGF2BP1 was identified as a direct binding partner of lncRNA MALAT1, highlighting its pivotal role within this intricate network. Moreover, we determined the optimal IGF2BP1 concentration (50 ng/ml) as a potent enhancer of osteogenesis, effectively countering the inhibition induced by si-MALAT1. Furthermore, in vivo experiments utilizing rat calvarial defects provided compelling evidence, solidifying lncRNA MALAT1’s crucial role in bone formation.

Conclusions

Our study reveals the regulatory network involving lncRNA MALAT1, miR-124-3p, and IGF2BP1 in PDLSCs’ osteogenic differentiation.

Clinical relevance

These findings enhance our understanding of lncRNA-mediated osteogenesis, offering potential therapeutic implications for periodontal tissue regeneration and the treatment of bone defects.

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Data availability

No datasets were generated or analysed during the current study.

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Funding

This study was financially supported by grants from the National Natural Science Foundation of China, Changchun, China (No.82370934).

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Authors and Affiliations

  1. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, 130021, China

    Nan Gu, Lingfeng Li, Xin Sui & Zhihui Liu

  2. Department of Prosthodontics, Hospital of Stomatology, Jilin University, Qinghua Road No.1500, Changchun, 130021, People’s Republic of China

    Nan Gu, Lingfeng Li, Xin Sui & Zhihui Liu

  3. Department of Stomatology, The First Hospital of Jilin University, Changchun, 130021, China

    Yao Wang

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  1. Nan Gu
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  2. Yao Wang
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  3. Lingfeng Li
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  4. Xin Sui
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  5. Zhihui Liu
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Contributions

Nan Gu: Conducted Experiments and Data Analysis.

Yao Wang: Conducted Literature Review and Manuscript Writing.

Lingfeng Li: Picture Production.

Xin Sui:Formal Analysis.

Zhihui Liu: Oversaw Project Design and Supervision.

Corresponding author

Correspondence to Zhihui Liu.

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Ethics approval

The experiment was conducted under the supervision of the Institutional Animal care and Use Committee of Jilin University. The number of permit was SY202104016.

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The authors declare that there is no conflict of interest.

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The authors declare no competing interests.

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Highlights

1. MALAT1 Enhances Osteogenesis: lncRNA MALAT1 positively regulates osteogenic differentiation in PDLSCs, highlighting its role in bone regeneration.

2. Unveiling the Regulatory Axis: miR-124-3p targets IGF2BP1 to modulate osteogenic potential, unveiling a novel regulatory axis in periodontal tissue repair.

3. Therapeutic Insights for Bone Defects: Insights into lncRNA-mediated osteogenesis provide potential avenues for innovative therapies in periodontal regeneration and bone defect management.

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Gu, N., Wang, Y., Li, L. et al. The mechanism of lncRNA MALAT1 targeting the miR-124-3p/IGF2BP1 axis to regulate osteogenic differentiation of periodontal ligament stem cells. Clin Oral Invest 28, 219 (2024). https://doi.org/10.1007/s00784-024-05616-3

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