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The regulation mechanism of LINC00707 on the osteogenic differentiation of human periodontal ligament stem cells

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Abstract

The osteogenic differentiation of periodontal ligament stem cells (PDLSCs) is important for periodontal tissue repair and regeneration. Long non-coding RNAs (lncRNAs) are key regulators of diverse biological processes. However, their roles in PDLSC osteogenic differentiation are still largely unknown. This study explored the effect of LINC00707 and its mechanism on the osteogenic differentiation of human PDLSCs. Results showed an increase in LINC00707 and forkhead box O1 (FOXO1) but a decrease in miR-490-3p during PDLSC osteogenic differentiation. LINC00707 and FOXO1 promoted osteogenic differentiation as evidenced by the formation of calcium nodules and the increase in osteogenic markers such as alkaline phosphatase, osteocalcin (OCN), and runt-related transcription factor 2 (Runx2). LINC00707 and FOXO1 knockdown exhibited opposite effects. Dual-luciferase reporter assay and qRT-PCR showed that LINC00707 can specially bind to miR-490-3p, which reversed the effect of LINC00707 on PDLSCs. MiR-490-3p inhibitor relieved the inhibiting effect of sh-LINC00707 on osteogenic differentiation. Further investigation revealed that LINC00707 can promote osteogenic differentiation by regulating FOXO1 expression through miR-490-3p sponging. Thus, the LINC00707/miR-490-3p/FOXO1 axis modulated PDLSC osteogenic differentiation and might be a promising therapeutic target for periodontal diseases.

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Funding

This work was supported by the Fujian Provincial Natural Science Foundation [Grant Number 2020J01646], Young Teacher Education and Research fund of Fujian Provincial Education Department [Grant Number JT180208], People’s Republic of China.

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Authors and Affiliations

  1. Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350002, Fujian, China

    Jianbin Guo & Minqian Zheng

  2. Institute of Stomatology & Research Center of Dental and Craniofacial Implants, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350002, Fujian, China

    Jianbin Guo

  3. Department of Orthodontics, Hospital of Stomatology, Fujian Medical University, No. 246 Yangqiao Zhong Road, Fuzhou, 350001, Fujian, China

    Minqian Zheng

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  1. Jianbin Guo
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  2. Minqian Zheng
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Correspondence to Minqian Zheng.

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The authors declare that they have no conflict of interest.

Ethical approval

hPDLSCs were obtained from pre-molars extracted from teenagers whose parents gave informed consent and were approved by the Ethics Committee of The Affiliated Stomatological Hospital of Fujian Medical University (20190027).

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Written informed consent for publication was obtained from all participants.

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Supplementary Information

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10735_2021_10029_MOESM1_ESM.jpg

Supplementary file1 Imaging of PDLSCs cultured in normal media after the third passage (P3) by optical microscopy (JPG 2237 kb)

10735_2021_10029_MOESM2_ESM.tif

Supplementary file2 Identification of the mesenchymal stem cell surface markers of PDLSCs by flow cytometry. All experiments were performed in triplicate, and results were expressed as means ± SD (TIF 16441 kb)

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Guo, J., Zheng, M. The regulation mechanism of LINC00707 on the osteogenic differentiation of human periodontal ligament stem cells. J Mol Histol 53, 13–26 (2022). https://doi.org/10.1007/s10735-021-10029-7

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  • DOI: https://doi.org/10.1007/s10735-021-10029-7

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