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Porcine circovirus genotypes and their copathogens in pigs with respiratory disease in southern provinces of Vietnam

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Abstract

This study was conducted to investigate the genetic diversity of porcine circovirus type 2 (PCV2) and its coinfecting pathogens in pigs with respiratory disease in Vietnam. Samples from 127 clinical cases were obtained from different southern provinces of Vietnam from January 2018 to January 2020 for PCR and sequence analysis. The infection rate of PCV2 was 78.8%, and the major pathogens found in coinfections with PCV2 were porcine reproductive and respiratory syndrome virus, Mycoplasma hyopneumoniae, and Haemophilus parasuis. Forty-three PCV2-positive clinical samples were selected for amplification and sequencing of the ORF2 region. Phylogenetic analysis of PCV2 ORF2 showed that five of the sequences belonged to PCV2b (11.6%) and 38 belonged to PCV2d (88.4%), indicating that PCV2d strains were predominant in southern provinces of Vietnam. Alignment of the predicted amino acid sequences of the PCV2 capsid protein revealed polymorphic sites in the antibody recognition regions. This study demonstrates the prevalence of the PCV2d genotype in southern Vietnam and presents a comprehensive overview of the coinfecting pathogens associated with PCV2 in young pigs with respiratory disease.

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Acknowledgements

This research was co-supported by Veterinary Hospital, HCMC University of Nonglam and Boehringer Ingelheim Vietnam through research facilities and research funds. The authors also express thanks to the technical personnel of Veterinary Hospital, pig production companies, and several faculty students who spent much time and energy to take part in this study.

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Author notes

  1. Phat Xuan Dinh and Minh Nam Nguyen are co-first authors.

Authors and Affiliations

  1. Department of Infectious Diseases and Veterinary Public Health, Faculty of Animal Science and Veterinary Medicine, Nong Lam University HCMC, Thu Duc district, Ho Chi Minh, Vietnam

    Hien The Nguyen, Dai Tan Vo, Hien Thanh Le, Nam Thi Thu Nguyen, Toan Tat Nguyen & Duy Tien Do

  2. Biotechnology Department, Nong Lam University HCMC, Linh Trung ward, Thu Duc district, Ho Chi Minh, Vietnam

    Phat Xuan Dinh

  3. School of Medicine, Vietnam National University Ho Chi Minh City, Linh Trung ward, Thu Duc district, Ho Chi Minh, Vietnam

    Minh Nam Nguyen

  4. Boehringer Ingelheim Vietnam, 39 Le Duan Street, District 1, Ho Chi Minh, Vietnam

    Vu Hoang Tran & Quy Dinh Tran

  5. Sanphar Vietnam Co. Ltd., 59, Xuan Thuy Street, Thao Dien Ward, District 2, Ho Chi Minh, Vietnam

    Kim Hoang Dang

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Contributions

Conceived of or designed study: VHT, DTV, HTL, NTTN, TTN, DTD. Performed research: MNN, PXD, HTN, VHT, QDT, KHD, DTD. Contributed new methods or models: PXD, DTV, TTN, DTD. Wrote the paper: PXD, MNN, DTD.

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Correspondence to Duy Tien Do.

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Dinh, P.X., Nguyen, M.N., Nguyen, H.T. et al. Porcine circovirus genotypes and their copathogens in pigs with respiratory disease in southern provinces of Vietnam. Arch Virol 166, 403–411 (2021). https://doi.org/10.1007/s00705-020-04878-y

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