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Efficient separation of phosphopeptides employing a Ti/Nb-functionalized core-shell structure solid-phase extraction nanosphere

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Abstract

A strategy for effectively enriching global phosphopeptides was successfully developed by using ammonia methyl phosphate (APA) as a novel chelating ligand and Ti4+ and Nb5+ as double functional ions (referred to as Fe3O4@mSiO2@APA@Ti4+/Nb5+). With the advantage of large specific surface area (151.1 m2/g), preeminent immobilized ability for metal ions (about 8% of total atoms), and unbiased enrichment towards phosphopeptides, Fe3O4@mSiO2@APA@Ti4+/Nb5+ displays high selectivity (maximum mass ratio β-casein to BSA is 1:1500), low limit of detection (LOD, as low as 0.05 fmol), good relative standard deviation (RSD, lower than 7%), recovery rate of 87% (18O isotope labeling method), outstanding phosphopeptide loading capacity (330 μg/mg), and at least five times re-use abilities. In the examination of the actual sample, 24 phosphopeptides were successfully detected in saliva and 4 phosphopeptides were also selectively extracted from human serum. All experiments have shown that Fe3O4@mSiO2@APA@Ti4+/Nb5+ exhibits exciting potential in view of the challenge of low abundance of phosphopeptides.

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Funding

This work is supported by Zhejiang Natural Science Foundation (LQ19C050002), State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1901), National Natural Science Foundation of China (511903050), and the K. C. Wong Magna Fund in Ningbo University.

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China

    Bin Liu, Baichun Wang, Yinghua Yan, Keqi Tang & Chuan-Fan Ding

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  1. Bin Liu
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  2. Baichun Wang
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  3. Yinghua Yan
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  4. Keqi Tang
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  5. Chuan-Fan Ding
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Correspondence to Yinghua Yan.

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Liu, B., Wang, B., Yan, Y. et al. Efficient separation of phosphopeptides employing a Ti/Nb-functionalized core-shell structure solid-phase extraction nanosphere. Microchim Acta 188, 32 (2021). https://doi.org/10.1007/s00604-020-04652-6

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  • DOI: https://doi.org/10.1007/s00604-020-04652-6

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