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Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides

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Abstract

An anion exchange solid-phase sorbent is described. Chitosan coated magnetite nanoparticles were modified with polyethylenimine which is positively charged at pH 3 and therefore can be used for the magnet-supported enrichment of phosphopeptides which are negatively charged at this pH value. A 2-step strategy was used to synthesize the sorbent. The materials were characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry and magnetic moment analysis. The anion exchanger was applied to extract phosphopeptides from a β-casein digest. Characteristic analytical figures include (a) a loading buffer of pH 3, (b) and elution buffer of pH 11, (c) a loading time of 5 min, (d) good selectivity (the β-casein to BSA ratio is 1:1000), and (e) excellent sensitivity (1 fmol). The optimized method was applied to egg yolk digest, non-fat milk digest, and diluted human serum.

Schematic representation of synthesis of PEI@chitosan@Fe3O4 nanoparticles, and of the enrichment of phosphopeptides by magnetic solid phase extraction prior to the determination of the peptides by MALDI-MS analysis.

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Acknowledgements

The authors acknowledge Higher Education Commission (HEC) of Pakistan for financial support. Furthermore, authors declare no conflict of interest.

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Authors and Affiliations

  1. Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60000, Pakistan

    Dilshad Hussain, Muhammad Naeem Ashiq & Muhammad Najam-ul-Haq

  2. HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan

    Dilshad Hussain & Syed Ghulam Musharraf

  3. Department of Biochemistry, Bahauddin Zakariya University, Multan, 60000, Pakistan

    Batool Fatima

  4. Department of Chemistry, The Women University, Multan, 60000, Pakistan

    Adeela Saeed & Fahmida Jabeen

  5. Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Muhammad Najam-ul-Haq

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  1. Dilshad Hussain
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Correspondence to Muhammad Najam-ul-Haq.

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Hussain, D., Musharraf, S.G., Fatima, B. et al. Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides. Microchim Acta 186, 852 (2019). https://doi.org/10.1007/s00604-019-3971-y

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