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Development of diamond-lanthanide metal oxide affinity composites for the selective capture of endogenous serum phosphopeptides

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Abstract

Development of affinity materials for the selective enrichment of phosphopeptides has attracted attention during the last decade. In this work, diamond-lanthanum oxide and diamond-samarium oxide composites have been fabricated via the hydrothermal method. The composites are characterized by scanning electron microscopy (SEM), energy dispersive X-Ray spectroscopy (EDAX), and atomic force microscopy (AFM). The analyses confirm the size and composition of the nanocomposites. They have been applied to selectively capture phosphorylated peptides from standard proteins (β-casein and BSA). Selectivity is calculated as 1:3000 and 1:1500 while sensitivity down to 1 and 20 fmol for diamond-lanthanum oxide and diamond-samarium oxide nanocomposites, respectively. Enrichment efficiency has also been evaluated for non-fat milk digest where 18 phosphopeptides are enriched. Total of 213 and 187 phosphopeptides are captured from tryptic digest of HeLa cells extracted proteins by diamond-lanthanum oxide and diamond-samarium oxide, respectively. Finally, human serum, without any pre-treatment, is applied and nanocomposites capture the endogenous serum phosphopeptides.

Capture of phosphopeptides through nanocomposites

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Acknowledgments

This work is supported by the Higher Education Commission (HEC) of Pakistan. Muhammad Naeem Ashiq, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan, is acknowledged for the useful discussions regarding the nanocomposites synthesis.

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Authors and Affiliations

  1. Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Dilshad Hussain & Muhammad Najam-ul-Haq

  2. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan

    Syed Ghulam Musharraf

Authors
  1. Dilshad Hussain
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  2. Syed Ghulam Musharraf
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  3. Muhammad Najam-ul-Haq
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Correspondence to Muhammad Najam-ul-Haq.

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The authors declare that they have no conflict of interest.

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Human serum samples were collected from the healthy volunteers after taking their written consent and the approval by the ethical committee of the Institute.

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Hussain, D., Musharraf, S.G. & Najam-ul-Haq, M. Development of diamond-lanthanide metal oxide affinity composites for the selective capture of endogenous serum phosphopeptides. Anal Bioanal Chem 408, 1633–1641 (2016). https://doi.org/10.1007/s00216-015-9272-3

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  • DOI: https://doi.org/10.1007/s00216-015-9272-3

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