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Nonlinear polymer brush modified magnetic nanoparticles in phosphopeptides enrichment

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Abstract

Magnetic nanoparticles attached to hydrophilic polymer brushes containing immobilized Fe3+ ions were synthesized and used as a new strategy for phosphopeptide enrichment from digested tryptic proteins. To test the performance of nanoparticles in phosphopeptide enrichment, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used. Magnetic nanoparticles with the following features such as high adsorption capacity (about 360 mg/g), easy control and transfer by external magnetic field (high magnetic saturation, about 50 emu/g), excellent phosphopeptide recovery (93.4%) and no shadow effect (due to their non-porous structure), were synthesized. It has been shown that the presence of other proteins in high concentrations does not affect on the performance of nanoparticles. Furthermore, the lowest concentration of digested β-casein phosphopeptides detectable by nanoparticles was determined to be approximately 0.5 fmol µL− 1. The function of synthesized nanoparticles to enrich phosphopeptides in complex biological samples was also demonstrated by isolating four endogenous phosphopeptides from human serum.

Graphical Abstract

A dispersion containing Fe-MNPs is added to a phosphopeptide solution. Then, using a magnet, particles containing phosphopeptides are separated from the solution

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Acknowledgements

We appreciate Iran Polymer and Petrochemical Institute and the Department of Chemistry of Islamic Azad University, Central Tehran Branch for their support.

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Authors and Affiliations

  1. Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran

    Zahra Shirzadi, Mahshid Nikpour Nezhati & Homayon Ahmad Panahi

  2. Faculty of Polymer Science, Iran Polymer and Petrochemical Institute, Tehran, Iran

    Habibollah Baharvand

  3. Department of Biological Science, Tarbiat Modares University, Tehran, Iran

    Reza H. Sajedi

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  1. Zahra Shirzadi
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  2. Habibollah Baharvand
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  3. Mahshid Nikpour Nezhati
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Z. Shirzadi, H. Baharvand, M. Nikpour Nezhati, H. Ahmad Panahi and R. H. Sajedi. The first draft of the manuscript was written by Zahra Shirzadi and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Habibollah Baharvand.

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Shirzadi, Z., Baharvand, H., Nezhati, M.N. et al. Nonlinear polymer brush modified magnetic nanoparticles in phosphopeptides enrichment. Colloid Polym Sci 302, 911–923 (2024). https://doi.org/10.1007/s00396-024-05244-z

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