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A Cu2(OH)3Cl-CeO2 nanocomposite with peroxidase-like activity, and its application to the determination of hydrogen peroxide, glucose and cholesterol

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Abstract

A nanomaterial of the chemical composition Cu2(OH)3Cl-CeO2 and with a large surface area is shown to be a viable peroxidase mimetic. It was synthesized by co-precipitation of an aqueous solution containing Ce(III) chloride, Cu(II) chloride and hexamethylenetetramine by adding an ionic liquid. The material was characterized by scanning electron microscopy and X-ray powder diffractometry. The composite possesses peroxidase-like activity and catalyzes the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine by H2O2 to produce a blue product. Based on this finding, a simple, rapid and selective colorimetric method was worked out for the determination of glucose and cholesterol by using the respective oxidases and by quantifying the H2O2 formed. Both glucose and cholesterol can be determined by this method at levels as low as 50 µM.

We found that the newly synthesized Cu2(OH)3Cl-CeO2 composite material possesses intrinsic peroxidase-like activity, and this finding was applied to design a rapid and selective colorimetric assay for H2O2, glucose and cholesterol.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 21375079, 51402175) and Project of Development of Science and Technology of Shandong Province, China (No. 2013GZX20109).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, 271018, China

    Nan Wang, Jianchao Sun, Lijian Chen, Hai Fan & Shiyun Ai

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  1. Nan Wang
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  2. Jianchao Sun
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  3. Lijian Chen
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  4. Hai Fan
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  5. Shiyun Ai
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Correspondence to Hai Fan or Shiyun Ai.

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Wang, N., Sun, J., Chen, L. et al. A Cu2(OH)3Cl-CeO2 nanocomposite with peroxidase-like activity, and its application to the determination of hydrogen peroxide, glucose and cholesterol. Microchim Acta 182, 1733–1738 (2015). https://doi.org/10.1007/s00604-015-1506-8

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  • DOI: https://doi.org/10.1007/s00604-015-1506-8

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