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Variation among matsutake ectomycorrhizae in four clones of Pinus sylvestris

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Abstract

Tricholoma matsutake is an ectomycorrhizal fungus that forms commercially important mushrooms in coniferous forests. In this study, we explored the ability of T. matsutake to form mycorrhizae with Pinus sylvestris by inoculating emblings produced through somatic embryogenesis (SE) in an aseptic culture system. Two months after inoculation, clones with less phenolic compounds in the tissue culture phase formed mycorrhizae with T. matsutake, while clones containing more phenols did not. Effects of inoculation on embling growth varied among clones; two of the four tested showed a significant increase in biomass and two had a significant increase in root density. In addition, results suggest that clones forming well-developed mycorrhizae absorbed more Al, Fe, Na, P, and Zn after 8 weeks of inoculation. This study illustrates the value of SE materials in experimental work concerning T. matsutake as well as the role played by phenolic compounds in host plant response to infection by mycorrhizal fungi.

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Acknowledgments

We are grateful to the Laboratory of Forest Botany, University of Tokyo for providing the Japanese isolate, A. Siika for help with the illustration, V. Miettinen for help with microscopy sections preparing, and M. Hardman for checking the language. This research was supported by the Foundation for Forest Breeding in Finland and European Regional development Fund of EU for the project “Vegetative propagation–knowhow and technology for enhancing bioeconomy.”

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Authors and Affiliations

  1. Finnish Forest Research Institute, PL 18, FI-01301, Vantaa, Finland

    Lu-Min Vaario & Arja Tervahauta

  2. Finnish Forest Research Institute, Finlandiantie 18, FI-58450, Punkaharju, Finland

    Jinrong Lu & Tuija Aronen

  3. University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland

    Arto Koistinen

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  1. Lu-Min Vaario
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  2. Jinrong Lu
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Correspondence to Lu-Min Vaario.

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Vaario, LM., Lu, J., Koistinen, A. et al. Variation among matsutake ectomycorrhizae in four clones of Pinus sylvestris . Mycorrhiza 25, 195–204 (2015). https://doi.org/10.1007/s00572-014-0601-8

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