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Phenolics of mycorrhizas and non-mycorrhizal roots of Norway spruce

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Abstract

The occurrence and amount of soluble and insoluble phenolics in mycorrhizal and non-mycorrhizal roots of Picea abies (L.) Karst, were investigated, p-Hydroxybenzoic acid glucoside, picein, piceatannol and its glucoside, isorhapontin, catechin and ferulic acid could be identified by high-performance liquid chromatography in mycorrhizas of Picea abies-Lactarius deterrimus and Picea abies-Laccaria amethystea. Both types were collected from axenic cultures and the latter also from a spruce stand. The same phenolics occurred in non-mycorrhizal short roots from sterile cultures. However, the amounts of p-hydroxybenzoic acid glucoside, picein, catechin and cell wall-bound ferulic acid were considerably reduced in mycorrhizas from axenic culture, whereas the hydroxystilbenes piceatannol, its glucoside and worhapontin were not significantly reduced. Pure mycelia of Laccaria amethystea (Bull.) Murr, and Lactarius deterrimus Gröger were also analysed for phenolic compounds. Both fungal species contained none of the identified phenolics. The results are discussed with respect to mycorrhization in different mycorrhizal types.

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Authors and Affiliations

  1. Institut für Botanik, Spezielle Botanik, Mykologie, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 1, D-7400, Tübingen

    Babette Münzenberger, Ingrid Kottke & Franz Oberwinkler

  2. Institut für Pharmazeutische Biologie der Technischen Universität Braunschweig, Mendelssohnstrasse 1, D-3300, Braunschweig, Germany

    Jürgen Heilemann & Dieter Strack

Authors
  1. Babette Münzenberger
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  2. Jürgen Heilemann
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  3. Dieter Strack
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  4. Ingrid Kottke
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  5. Franz Oberwinkler
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Additional information

We are grateful to the Deutsche Forschungsgemeinschaft (Schwerpunktprogramm “Physiologie der Bäume”) and the Fonds der Chemischen Industrie for financal support. We thank Dr. V. Wray (GBF, Braunschweig, FRG) for linguistic advice.

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Münzenberger, B., Heilemann, J., Strack, D. et al. Phenolics of mycorrhizas and non-mycorrhizal roots of Norway spruce. Planta 182, 142–148 (1990). https://doi.org/10.1007/BF00239996

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