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Ectomycorrhization of Tricholoma matsutake and two major conifers in Finland—an assessment of in vitro mycorrhiza formation

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Abstract

This study aimed to test the ability of Tricholoma matsutake isolates to form mycorrhizas with aseptic seedlings of Pinus sylvestris L. and Picea abies (L.) Karst. Germinated seedlings of Scots pine and Norway spruce were separately inoculated with either isolates originating from Finland or Japan. Eight months after inoculation, the Finnish isolate had formed a sheath and Hartig net on both host species. Ectomycorrhizal Scots pine seedlings inoculated with the Finnish isolate showed the same shoot height and dry mass as the controls. Ectomycorrhizal Norway spruce seedlings inoculated with the Finnish isolate had similar shoot height but slightly less dry mass than the control seedlings. For both tree species, inoculation with the Finnish isolate resulted in reduced total nitrogen content per seedling, but carbon content was unaffected. Inoculation with the Japanese isolate resulted in an initial Hartig net-like structure in pine but not in spruce. No typical Hartig net was observed on either tree species. Furthermore, seedlings of both species inoculated with the Japanese isolate showed significantly reduced growth, dry mass, nitrogen, and carbon content per seedling and shoot height (in spruce) compared to the controls. This study documents and describes the in vitro ectomycorrhization between T. matsutake and Scots pine or Norway spruce and the variable mycorrhizal structures that matsutake isolates can form.

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Acknowledgements

We are grateful to the Laboratory of Forest Botany, University of Tokyo for providing the Japanese T. matsutake isolate, A. Siika for help with the illustration, and M. Hardman for checking the language. We also thank the reviewers for the valuable comments. This research was supported by the Foundation for Research of Natural Resources in Finland. The financial support for J. Heinonsalo by the Academy of Finland project number 121915 is greatly acknowledged.

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Authors and Affiliations

  1. Finnish Forest Research Institute, Vantaa Research Unit, PL 18, 01301, Vantaa, Finland

    Lu-Min Vaario & Taina Pennanen

  2. Finnish Forest Research Institute, Parkano Research Unit, 39700, Parkano, Finland

    Tytti Sarjala & Eira-Maija Savonen

  3. Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland

    Jussi Heinonsalo

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  1. Lu-Min Vaario
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  2. Taina Pennanen
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  3. Tytti Sarjala
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  4. Eira-Maija Savonen
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  5. Jussi Heinonsalo
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Correspondence to Lu-Min Vaario.

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Vaario, LM., Pennanen, T., Sarjala, T. et al. Ectomycorrhization of Tricholoma matsutake and two major conifers in Finland—an assessment of in vitro mycorrhiza formation. Mycorrhiza 20, 511–518 (2010). https://doi.org/10.1007/s00572-010-0304-8

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