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Fed-batch polyhydroxybutyrate production by Paraburkholderia sacchari from a ternary mixture of glucose, xylose and arabinose

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Abstract

Polyhydroxybutyrate (PHB) is a biodegradable bioplastic that is comparable with many petroleum-based plastics in terms of mechanical properties and is highly biocompatible. Lignocellulosic biomass conversion into PHB can increase profit and add sustainability. Glucose, xylose and arabinose are the main monomer sugars derived from upstream lignocellulosic biomass processing. The sugar mixture ratios may vary greatly depending on the pretreatment and enzymatic hydrolysis conditions. Paraburkholderia sacchari DSM 17165 is a bacterium strain that can convert all three sugars into PHB. In this study, fed-batch mode was applied to produce PHB on three sugar mixtures (glucose:xylose:arabinose = 4:2:1, 2:2:1, 1:2:1). The highest PHB concentration produced was 67 g/L for 4:2:1 mixture at 41 h corresponding to an accumulation of 77% of cell dry weight as PHB. Corresponding sugar conversion efficiency and productivity were 0.33 g PHB/g sugar consumed and 1.6 g/L/h, respectively. The results provide references for process control to maximize PHB production from real sugar streams derived from corn fibre.

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Acknowledgements

The authors would like to thank the University of Nebraska Agricultural Research Division and the Nebraska Corn Board under contract 88-R-1920-44 for funding this research.

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  1. Mark R. Wilkins

    Present address: , 211 Chase Hall, 3605 Fair St, Lincoln, 68583, NE, USA

Authors and Affiliations

  1. Department of Biological Systems Engineering, The University of Nebraska-Lincoln, Lincoln, 68583, NE, USA

    Mengxing Li & Mark R. Wilkins

  2. Department of Statistics, The University of Nebraska-Lincoln, Lincoln, 68583, NE, USA

    Mengxing Li

  3. Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, 68588, NE, USA

    Mark R. Wilkins

  4. Industrial Agricultural Products Center, University of Nebraska-Lincoln, Lincoln, 68583, NE, USA

    Mark R. Wilkins

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Li, M., Wilkins, M.R. Fed-batch polyhydroxybutyrate production by Paraburkholderia sacchari from a ternary mixture of glucose, xylose and arabinose. Bioprocess Biosyst Eng 44, 185–193 (2021). https://doi.org/10.1007/s00449-020-02434-1

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