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Overexpression of a novel SbMYB15 from Salicornia brachiata confers salinity and dehydration tolerance by reduced oxidative damage and improved photosynthesis in transgenic tobacco

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Abstract

Main conclusion

SbMYB15, R2R3-type MYB was induced by the different stresses, and conferred stress tolerance in transgenic tobacco by regulating the expression of stress-responsive genes.

MYBs are the master regulators of various metabolic processes and stress responses in plants. In this study, we functionally characterised a R2R3-type SbMYB15 transcription factor (TF) from the extreme halophyte Salicornia brachiata. The SbMYB15 acts as a transcriptional activator. Transcriptional analysis showed that SbMYB15 transcript was strongly upregulated in red shoots and was also induced by different stresses; however, its expression remained unchanged with ABA. Overexpression of SbMYB15 in tobacco significantly improved salinity and dehydration tolerance. The enhanced tolerance of the transgenic plants was defined by the changes in chlorophyll, malondialdehyde (MDA), proline, total soluble sugar and total amino acid contents. The transgenic plants exhibited a higher membrane stability and reduced electrolyte leakage, H2O2 and O −2 content compared to the wild type (WT). With ionic stress, transgenics showed a low Na+ and a high K+ content. In the transgenic plants, the expression of stress-responsive genes such as LEA5, ERD10D, PLC3, LTP1, HSF2, ADC, P5CS, SOD and CAT was enhanced in the presence of salinity, dehydration and heat. Exposure to gradual salinity and dehydration resulted in an increased stomatal conductance, water use efficiency, photosynthesis rate, photochemical quenching and reduced transpiration rate. Thus, SbMYB15 served as an important mediator of stress responses regulating different stress signalling pathways, leading to enhanced stress tolerance.

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Abbreviations

ABA:

Abscisic acid

Ci:

Intercellular CO2 concentration

Ca:

Ambient CO2 concentration

ETR:

Photosynthetic electron transport rate

g:

Stomatal conductance

MDA:

Malondialdehyde

NPQ:

Non-photochemical fluorescence quenching

PEG:

Polyethylene glycol

SA:

Salicylic acid

TF:

Transcription factor

VA:

Transgenic plants transformed with pCAMBIA 1301

WUE:

Water use efficiency

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Acknowledgments

CSIR-CSMCRI Communication No. 019/2015. The authors are thankful to Department of Science and Technology (DST) and Council of Scientific and Industrial Research (CSIR), New Delhi, India for financial assistance. P.S. Shukla is thankful to AcSIR for enrolment in Ph.D. and CSIR for financial support.

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Authors and Affiliations

  1. Division of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364 002, Gujarat, India

    Pushp Sheel Shukla, Kapil Gupta, Parinita Agarwal & Pradeep K. Agarwal

  2. Division of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364002, Gujarat, India

    Bhavanath Jha

  3. Academy of Scientific and Innovative Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364 002, Gujarat, India

    Pushp Sheel Shukla, Bhavanath Jha & Pradeep K. Agarwal

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  1. Pushp Sheel Shukla
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  2. Kapil Gupta
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Correspondence to Pradeep K. Agarwal.

Electronic supplementary material

Below is the link to the electronic supplementary material.

425_2015_2366_MOESM1_ESM.pptx

Supplementary material 1 Fig. S1 Schematic representation of gradual exposure to treatments of N. tabacum WT and transgenic lines in the greenhouse(PPTX 1055 kb)

425_2015_2366_MOESM2_ESM.pptx

Supplementary material 2 Fig. S2 Sequence logos of R2–R3 repeats of SbMYB15. The overall height of each stack indicates the conservation of the sequence at particular position. The height of letters within each stack represents the relative frequency of the corresponding amino acid. The triangle indicates the positions of the conserved tryptophan (W) and phenylalanine (F) amino acids, which are identical in other MYB proteins (PPTX 284 kb)

425_2015_2366_MOESM3_ESM.pptx

Supplementary material 3 Fig. S3 Nucleotide and deduced amino acid sequences of the SbMYB15 gene. R2 and R3 repeats are marked by black arrows. Conserved tryptophan residues are shown by (*). The presence of SANT domains in SbMYB15 is demarcated by a red box. Linker region of R2 and R3 repeat is marked by a red dotted line. Blue and brown arrows represent a serine- and histidine-rich region, respectively. Green bars represent alpha helices and a red arrow represents the presence of a loop (PPTX 135 kb)

425_2015_2366_MOESM4_ESM.pptx

Supplementary material 4 Fig. S4 a Secondary structure prediction of SbMYB15 shows the presence of alpha helices, strands and coils. b Secondary structure composition. c Solvent accessibility of SbMYB15 protein (PPTX 205 kb)

Supplementary material 5 Fig. S5 Phylogenetic analysis SbMYB15 transcription factor (PPTX 90 kb)

425_2015_2366_MOESM6_ESM.pptx

Supplementary material 6 Fig. S6 Transactivation assay of SbMYB15. a Transformed yeast cells (AH109) with pGBKT7-SbMYB15 and pGBKT7 alone were grown on SD/-Trp/-His/-Ura medium. b Schematic representation of the plating. c Yeast cells transferred on filter paper showed β-galactosidase (encoded by LacZ gene) activity using X-gal staining (PPTX 137 kb)

425_2015_2366_MOESM7_ESM.pptx

Supplementary material 7 Fig. S7 a Diagrammatic representation of the pCAMBIA1301-35S:SbMYB15 construct. Screening of T1 transgenic plants. b–c PCR confirmation of T1 transgenics with SbMYB15 gene specific primers and hptII primers. d GUS staining of seedlings of N. tabacum WT (i), VA (pCAMBIA1301 alone) (ii), and 35S:SbMYB15 overexpressing transgenic lines (iii–vii). e GUS:NRA ratio for determination of copy number of transgene insertion in transgenics (PPTX 777 kb)

425_2015_2366_MOESM8_ESM.pptx

Supplementary material 8 Fig. S8 a Morphological analysis of N. tabacum transgenic lines (L-79, L-86, L-87, L-123, L-127, L-158 and VA) and WT plants in 0, 100 and 200 mM NaCl concentrations in hydroponic medium. b RT-PCR analysis of the three SbMYB15 representative overexpression lines. c Growth of N. tabacum WT and transgenic lines (L-87, L-123, L-127, L-158 and VA) in 0 and 10 % PEG in hydroponic medium (PPTX 754 kb)

Supplementary material 9 (DOCX 15 kb)

Supplementary material 10 (DOCX 15 kb)

Supplementary material 11 (DOCX 14 kb)

Supplementary material 12 (DOCX 20 kb)

Supplementary material 13 (DOCX 18 kb)

Supplementary material 14 (DOCX 19 kb)

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Shukla, P.S., Gupta, K., Agarwal, P. et al. Overexpression of a novel SbMYB15 from Salicornia brachiata confers salinity and dehydration tolerance by reduced oxidative damage and improved photosynthesis in transgenic tobacco. Planta 242, 1291–1308 (2015). https://doi.org/10.1007/s00425-015-2366-5

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