Abstract
In the agricultural areas of the Qinghai-Tibet Plateau, ultraviolet-B (UV-B) radiation varies widely. UV-B can affect the growth and development of plants. Turnip (Brassica rapa var. rapa) is an important traditional economic crop in the Qinghai-Tibet Plateau, but how turnip responds to UV-B and the complex molecular mechanisms underlying UV-B tolerance remains unclear. In this work, we found that landrace KTRG-B48b grew better and obtained a higher fresh weight of taproot in Lhasa and Kunming than landrace KTRG-B48a through common garden experiments. The phenotypic differences between the two landraces may be mechanism-based. Transcriptomes was used to analyze the differences between KTRG-B48a and KTRG-B48b. The relative expression levels of anthocyanin synthesis genes were upregulated significantly after UV-B treatment. In addition, KTRG-B48b produced less hydrogen peroxide and superoxide than KTRG-B48a. The high expression levels of anthocyanin-related genes can increase accumulation of anthocyanin, which can decrease reactive oxygen species accumulation and improved plant adaptation to the living environment of strong UV-B radiation on the Qinghai-Tibet Plateau. In this study, we proposed a model for turnip anthocyanin biosynthesis under strong UV-B radiation. Our results can provide an overview of the transcriptome response of turnip to UV-B tolerance, thus expanding our understanding the functions of anthocyanins biological in plants.
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Acknowledgements
The authors highly acknowledge Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences. We thank the editor and reviewers for providing valuable comments on the manuscript.
Funding
This work was supported by the Strategic Priority Research Program of Chinese Academy of Science (XDA2004010306), Science and Technology Program of Xizang Autonomous Region, China (XZ202001YD0008C), the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (2019QZKK0502) and the Key Program for Basic Research of Yunnan Province (2018FA052).
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YZ: Conceptualization, methodology, writing-original draft preparation, data curation and analysis; JCD: original draft preparation, data curation and analysis; TTN and CDW: Methodology; YQY and DNY: Data analysis; YWD: Writing-Reviewing; YPY: Reviewing and Supervision; XDS: Reviewing, Editing and Supervision.
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Zhang, Y., Du, J., Ni, T. et al. Comparative Transcriptome Analysis Reveals the Complex Molecular Mechanisms Underlying Ultraviolet-B Tolerance in Brassica rapa var. rapa. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11286-y
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DOI: https://doi.org/10.1007/s00344-024-11286-y