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Filamentation Temperature-Sensitive (FtsH); Key Player in Response to Multiple Environmental Stress Conditions and Developmental Stages in Potato

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Abstract

Biotic and abiotic stresses are among major factors limiting crop yields, and Filamentation temperature-sensitive (FtsH) genes are one of the key regulators of plant response to these stresses. The FtsH is an ATP-dependent metalloprotease in prokaryotes and eukaryotes. Due to the economic importance and cultivation of potato grown in the biotic and abiotic stress prone areas, identification and characterization of FtsH family members are crucially important. The present research was conducted to investigate FtsH family members in potato under stressful regime. To detect the FtsH genes in potato, we performed genome-wide analysis of FtsH genes in the Solanum tuberosum genome using the Ensemble Plant. All putative sequences were approved with the Pfam. Bioinformatics analysis was conducted using phylogenetic tree, gene structure, Transcription factor-binding site (TFBS) analysis, protein–protein interaction, and gene expression. The members of FtsH were categorized into eight groups. Some of the FtsH proteins were subcellularly located in the nucleus and chloroplast. The number of introns was ranged from 3 to 14 in gene family members. Totally, 33 TFBS including biotic and abiotic stress-responsive elements were found in FtsH promoter sequences. MYB and WRKY were the highest number among TFBS which were involved in abiotic and biotic stresses. Gene expression analysis revealed that the StFtsH2 and StFtsH5 had the highest gene expression, induced by abiotic and biotic stresses in all three tissues of stem, root, and leaves. It is expected that the StFtsH2 and StFtsH5 could be used in plant manipulation and breeding programs aimed for tolerance enhancement to abiotic (cold, heat, and high light) and biotic stresses [Potato virus Y (PVY), Potato virus X (PVX), Potato virus A (PVA), Potato virus X (PVA), and Potato virus S (PVS)].

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Abbreviations

AP2:

APETALA2

ATH:

AT hook

B3:

B3 DNA-binding domain (DBD)

bHLH:

Basic Helix–Loop–Helix

bZIP:

Basic Leucine Zipper

C2H2 ZF:

Cchhc domain of neural zinc finger factor

CSD:

Cold shock domain

Dof:

Dof (DNA binding with one finger) domain

EIN3:

Ethylene insensitive 3

FAR1:

FAR domain (Finger-Associated Repeats)

GATA:

GATA-binding factors

HB:

Homeodomain

LOB:

Lateral organ boundaries

MADS box:

The conserved domain originally discovered in four genes (MCM1, AG, DEF A, and SRF)

Myb:

Myeloblastosis

NAC:

No apical meristem [NAM], Arabidopsis transcription activation factor [ATAF], and cup-shaped cotyledon [CUC])-domain transcription factor gene

SBP:

SQUAMOSA-pROMOTER-BINDING PROTEIN

STK:

Storekeeper

TCP:

Teosinte branched 1 (tb1, Zea mays (Maize)), cycloidea (cyc) (Antirrhinum majus) (Garden snapdragon) and PCF in rice (Oryza sativa)

WRKY:

WRKY transcription factor family

ZF-HD:

Zinc finger homeodomain

Trihelix:

Helix–loop–helix–loop–helix

SRS:

SHORT INTERNODES-related sequence

SOX:

SRY-related high-mobility group box

WRC:

Trp-Arg-Cys

WOX:

WUSCHEL-related homeobox

BES1:

BRI1-EMSSUPPRESSOR1

VOZ:

VASCULAR PLANT ONE-ZINC FINGER

NF-YB:

Nuclear Transcription Factor Y Subunit Beta

TCR:

Transcription-coupled repeat

MADF:

Mothers against Dpp factor

ARF:

Auxin-responsive element

HSF:

Heat Shock Factor

HD-ZIP:

Homeodomain-leucine zipper

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  1. Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

    Zahra Hajibarat & Abbas Saidi

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  1. Zahra Hajibarat
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Hajibarat, Z., Saidi, A. Filamentation Temperature-Sensitive (FtsH); Key Player in Response to Multiple Environmental Stress Conditions and Developmental Stages in Potato. J Plant Growth Regul 42, 4223–4239 (2023). https://doi.org/10.1007/s00344-022-10885-x

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