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Enhancement of Drought Tolerance in Transgenic Arabidopsis thaliana Plants Overexpressing Chickpea Ca14-3-3 Gene

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Abstract

Plant 14-3-3 proteins are highly conserved, phospho-binding proteins, ubiquitous in nature that control major cellular functions. The genes encoding 14-3-3 family proteins are reported for involvement in plant stress tolerance. Therefore, we here studied the function of chickpea (Cicer arietinum L.) 14-3-3-like protein B with increased expression under drought stress in Arabidopsis thaliana. Compared to wild type, Ca-14-3-3-like protein B overexpressing lines showed a high rate of germination, more biomass, and increased root length at the seedling stage under osmotic stress caused by both polyethylene glycol (PEG-6000) and mannitol. In addition, chlorophyll content, proline content, relative water content (RWC), antioxidant enzyme activities, net photosynthesis, and water use efficiency were also higher in transgenic lines as compared to WT after drought stress. Staining with DAB and NBT indicated that transgenic lines accumulated less H2O2 than WT plants. Furthermore, Ca-14-3-3-like protein B overexpression also upregulated the transcript level of the certain stress-responsive genes (RD20, PDF1, and P5CS1) and of genes involved in lignin biosynthesis (PAL and CAD). Overall it can be concluded that Ca-14-3-3-like protein B improves the drought tolerance when overexpressed in Arabidopsis plants and the gene thus appears to be a promising candidate for crop improvement efforts.

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Acknowledgements

The authors acknowledge the Director, CSIR National Botanical Research Institute for providing facilities and support during the study. This work is supported by the CSIR-Network (MLP0048 and MLP0049) and an in-house project (OLP109) funded by the Council of Scientific and Industrial Research, New Delhi, India.

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Authors and Affiliations

  1. Microbial Technology Division, Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India

    Swati Gupta, Sankalp Misra, Manoj Kumar, Shashank Kumar Mishra, Shalini Tiwari,  Anshu, Lalit Agrawal & Puneet Singh Chauhan

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Swati Gupta, Sankalp Misra, Shiv Narayan & Puneet Singh Chauhan

  3. School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Shalini Tiwari

  4. Plant Physiology Laboratory, CSIR-National Botanical Research Institute, Lucknow, India

    Shiv Narayan

  5. Department of Agriculture and Allied Sciences, Doon Business School, Dehradun, 248001, India

    Lalit Agrawal

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  1. Swati Gupta
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PSC and LA conceived and co-ordinated the study. SG, SM, MK, and AA carried out the experiments and analyzed the data. SKM, ST, and SN assisted in experiments and discussed the results. SG, ST, SM, LA, and PSC wrote and edited the manuscript.

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Gupta, S., Misra, S., Kumar, M. et al. Enhancement of Drought Tolerance in Transgenic Arabidopsis thaliana Plants Overexpressing Chickpea Ca14-3-3 Gene. J Plant Growth Regul 42, 1544–1557 (2023). https://doi.org/10.1007/s00344-022-10639-9

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