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Expression of TaGF14b, a 14-3-3 adaptor protein gene from wheat, enhances drought and salt tolerance in transgenic tobacco

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Abstract

Main conclusion

TaGF14b enhances tolerance to multiple stresses through ABA signaling pathway by altering physiological and biochemical processes, including ROS-scavenging system, stomatal closure, compatible osmolytes, and stress-related gene expressions in tobaccos.

The 14-3-3 proteins are involved in plant growth, development, and in responding to abiotic stresses. However, the precise functions of 14-3-3s in responding to drought and salt stresses remained unclear, especially in wheat. In this study, a 14-3-3 gene from wheat, designated TaGF14b, was cloned and characterized. TaGF14b was upregulated by polyethylene glycol 6000, sodium chloride, hydrogen peroxide, and abscisic acid (ABA) treatments. Ectopic expression of TaGF14b in tobacco conferred enhanced tolerance to drought and salt stresses. Transgenic tobaccos had longer root, better growth status, and higher relative water content, survival rate, photosynthetic rate, and water use efficiency than control plants under drought and salt stresses. The contribution of TaGF14b to drought and salt tolerance relies on the regulations of ABA biosynthesis and ABA signaling, as well as stomatal closure and stress-related gene expressions. Moreover, TaGF14b expression could significantly enhance the reactive oxygen species (ROS) scavenging system to ameliorate oxidative damage to cells. In addition, TaGF14b increased tolerance to osmotic stress evoked by drought and salinity through modifying water conservation and compatible osmolytes in plants. In conclusion, TaGF14b enhances tolerance to multiple abiotic stresses through the ABA signaling pathway in transgenic tobaccos by altering physiological and biochemical processes.

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Abbreviations

ABF:

ABA-responsive element-binding transcription factor

CAT:

Catalase

DAB:

3,3′-Diaminobenzidine

DREB3:

Dehydration-responsive element-binding protein

DPI:

Diphenyleneiodonium

GFP:

Green fluorescent protein

LTP1:

Lipid transfer protein

MDA:

Malondialdehyde

NBT:

Nitroblue tetrazolium

NCED1:

9-cis-Epoxycarotenoid dioxygenase 1

OE:

Overexpression

P5CS:

Pyrroline-5-carboxylate synthetase

Pn:

Photosynthetic rate

POD:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Tu:

Sodium tungstate

VC:

Vector control

WT:

Wild type

WUE:

Water use efficiency

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Acknowledgements

The work was supported by National Genetically Modified New Varieties of Major Projects of China (2016ZX08010004-004), the National Natural Science Foundation of China (Nos. 31771418, 31570261), and Key Project of Hubei Province (2017AHB041).

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  1. Yang Zhang, Hongyan Zhao, and Shiyi Zhou have contributed equally to this article.

Authors and Affiliations

  1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Yang Zhang, Hongyan Zhao, Yuan He, Qingchen Luo, Fan Zhang, Ding Qiu, Jialu Feng, Qiuhui Wei, Lihong Chen, Mingjie Chen, Junli Chang, Guangxiao Yang & Guangyuan He

  2. Hubei Key Laboratory of Purification and Application of Plant Anticancer Active Ingredients, School of Chemistry and Life Sciences, Hubei University of Education, Wuhan, 430205, China

    Shiyi Zhou

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Fig. S1

Subcellular localization of TaGF14b. The control pBI121-GFP (a) and recombined pBI121-TaGF14b-GFP (b) vectors were transiently expressed in onion epidermal cells and observed with fluorescence microscope, respectively (TIFF 14002 kb)

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Zhang, Y., Zhao, H., Zhou, S. et al. Expression of TaGF14b, a 14-3-3 adaptor protein gene from wheat, enhances drought and salt tolerance in transgenic tobacco. Planta 248, 117–137 (2018). https://doi.org/10.1007/s00425-018-2887-9

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