Abstract
Key message
Nicotiana sylvestris calcineurin B-like protein NsylCBL10 improves tolerance to high-salt stress through better maintenance of Na + balance.
Abstract
The calcineurin B-like (CBL) proteins represent a unique group of plant calcium sensors and play an important role in regulating the response of a plant cell to the stress. Although many studies have been made in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa) and poplar (Populus trichocarpa), the characterization and elucidation of the functions of CBLs in tobacco have not yet been reported. In this study, NsylCBL10, a CBL gene showing higher similarities to other CBL10 genes, was cloned from Nicotiana sylvestris. NsylCBL10 is expressed in most of the tobacco tissues, and the protein targets to the plasma membrane specifically. Over-expression of NsylCBL10 enhanced the salt tolerance of Arabidopsis wild type plants greatly, and rescued the high-salt-sensitive phenotype of Arabidopsis cbl10 mutant. The analysis of ion content indicated that over-expressing NsylCBL10 in plants is able to maintain a lower Na+/K+ ratio in roots and higher Na+/K+ ratio in shoots, compared with cbl10 mutant. The results suggest that NsylCBL10 might play an important role in response to high salinity stress in N. sylvestris, by keeping a better ionic homeostasis to reduce the damage of toxic ion to the plant cell.
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Acknowledgments
We are grateful to Associate Professor Yi Wang and Yongliang Zhang (College of Biological Sciences, China Agricultural University) for providing the AtCBL10 over-expression Arabidopsis material and the plasma membrane marker, respectively. We also give thanks to Professor Yongfeng Guo and Professor Yingzhen Kong (Tobacco Research Institute, Chinese Academy of Agricultural Sciences) for providing us valuable suggestions. This research was supported by Central Research Institute of Basic Public Welfare Funds of China (2012ZL058) and National Natural Science Foundation of China (31201489).
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Communicated by C.-H. Dong.
L. Dong and Q. Wang contributed equally to this work.
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Dong, L., Wang, Q., Manik, S.M.N. et al. Nicotiana sylvestris calcineurin B-like protein NsylCBL10 enhances salt tolerance in transgenic Arabidopsis . Plant Cell Rep 34, 2053–2063 (2015). https://doi.org/10.1007/s00299-015-1851-4
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DOI: https://doi.org/10.1007/s00299-015-1851-4