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Genome Analysis of Halomonas elongata Strain 153B and Insights Into Polyhydroxyalkanoate Synthesis and Adaptive Mechanisms to High Saline Environments

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Abstract

Species of the Halomonas genus are gram-negative, aerobic, moderately halophilic bacteria that synthesize polyhydroxyalkanoates (PHAs) and other high-value products that have a wide range of potential uses in the food, feed, cosmetics, pharmaceutical, and chemical sectors. Genome sequencing studies allow for the description and comparison of genetic traits with other strains and species, allowing for the exploration of the organism's potential, necessary to further biotechnology applications. Here, the genome of Halomonas elongata strain 153B was sequenced, its features compared to 5 other strains and 7 species, and a description of features for adaptations to hypersaline environments and bioproducts synthesis was done. Whole-genome analysis showed H. elongata 153B has more similar features to the reference strain H. elongata DSM 2581 compared to 4 other reported strains. Comparative genomics showed 2064 core genomic clusters between the strains and 666 singletons for strain 153B. Several genes in transport and signaling, osmoregulation, and oxidative stress that have roles in adaptation to environments with high osmolarity were also revealed. These appear to form an intricate network of overlapping systems carefully coordinated to bring about adaptation. H. elongata 153B genes for the synthesis of PHAs, ectoine, vitamins, and the degradation of drugs and aromatic compounds were described. The results will aid in the study of halophile physiology, provide a mine for valuable enzymes, and help speed up research for other biotechnology applications.

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Data Availability

The genomic sequence data in this study were deposited in the NCBI database.

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Acknowledgements

This study is based partly on the Ph.D. thesis of BM. Enuh. Besides, we thank Prof. Mutlu for providing the strain.

Funding

This work has been supported by Eskisehir Osmangazi University Scientific Research Projects Coordination Unit under grant number FDK-2022-2468.

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Authors and Affiliations

  1. Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Science, Eskisehir Osmangazi University, 26040, Eskisehir, Turkey

    Blaise Manga Enuh & Pınar Aytar Çelik

  2. Environmental Protection and Control Program, Eskişehir Osmangazi University, 26110, Eskişehir, Turkey

    Pınar Aytar Çelik

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  1. Blaise Manga Enuh
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  2. Pınar Aytar Çelik
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PAÇ and EBM developed and designed the study, EBM did the literature search and experiments and analysis, PAÇ revised the work critically. All authors read and approved the final manuscript.

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Correspondence to Pınar Aytar Çelik.

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Enuh, B.M., Aytar Çelik, P. Genome Analysis of Halomonas elongata Strain 153B and Insights Into Polyhydroxyalkanoate Synthesis and Adaptive Mechanisms to High Saline Environments. Curr Microbiol 80, 18 (2023). https://doi.org/10.1007/s00284-022-03115-w

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