Abstract
Biofilm-immobilized fermentation is a novel strategy that has been utilized in L-lysine fermentation. In this study, we describe a strategy for designing bioreactors for immobilized fermentation. We have constructed steel structures in which the carriers can be sewn, forming several star-like structures with different angles, and changing the ventilation robot to the aeration tray. In a 10-L bioreactor, this structure with 12 angles assisted the immobilized system to remedy the gap between free-cell and immobilized fermentation in the conversion rate. In a 50-L bioreactor, this enlarged structure with 16 angles illustrated a 4.61% higher conversion rate than the free-cell fermentation (67.75%) and increased the production by 28.56%. This successful case is the first step towards to industrial production of biofilm-based immobilized fermentation.
Key points
• The designed steel structure is useful for L-lysine immobilized fermentation in a 10-L bioreactor.
• The conversion rate of immobilized fermentation increased from 13.99 to 60.07% and is 1.03% higher than that of the free-cell fermentation.
• The conversion rate of the redesigned 50-L bioreactor is higher than that of free-cell fermentation.
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Change history
26 June 2023
A Correction to this paper has been published: https://doi.org/10.1007/s00253-023-12645-7
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Funding
This work was supported by the National Key R&D Program of China (Grant No. 2021YFC2101100); the National Natural Science Foundation of China (22178176): the National Key Research and Development Program of China (Grant No. 2018YFB1501705); the key program of the National Natural Science Foundation of China (Grant No. 21636003); the Outstanding Youth Foundation of China (Grant No. SBK2017010373); the Program for Changjiang Scholars and Innovative Research Team in University (IRT_14R28); Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM); the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD); Key Research and Development Program of Nanjing Jiangbei New Area (ZDYF20200220); the National Natural Science Foundation of China (Grant No.: 22178172).
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GM and WZ contributed equally to this work and should be considered as co-first authors. GM, QL, CZ, and YZ performed the fermentation experiments. WZ and YZ drafted and revised the manuscript. WS, NZ, and DL participated in the fermentation experiments and SEM assay. YC and WS participated in the design of the study and revised the manuscript. HY conceived of the study and participated in its design. All authors read and approved the final manuscript.
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Meng, G., Zhao, W., Liu, Q. et al. Strategy for the design of a bioreactor for L-lysine immobilized fermentation using Corynebacterium glutamicum. Appl Microbiol Biotechnol 106, 5449–5458 (2022). https://doi.org/10.1007/s00253-022-12103-w
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DOI: https://doi.org/10.1007/s00253-022-12103-w