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Colorimetric sensing strategy for detection of cysteine, phenol cysteine, and phenol based on synergistic doping of multiple heteroatoms into sponge-like Fe/NPC nanozymes

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Abstract

Nanozymes have both the high catalytic activity of natural enzymes and the stability and economy of mimetic enzymes. Research on nanozymes is rapidly emerging, and the continuous development of highly catalytic active nanozymes is of far-reaching significance. This work reports heteroatomic nitrogen (N) and phosphorus (P) double-doped mesoporous carbon structures and metallic Fe coordination generated sponge-like nanozymes (Fe/NPCs) with good peroxidase activity. On this basis, we constructed a highly sensitive colorimetric sensor with cysteine and phenol as simulated analytes using Fe/NPCs nanozymes, and the response limits reached 53.6 nM and 5.4 nM, respectively. Besides, the method has high accuracy in the detection of cysteine and phenol at low concentrations in serum and tap water, which lays a foundation for application in the fields of environmental protection and biosensors.

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Funding

This work was financially supported by the Natural Science Foundation of Beijing Municipality (no. 2192010 and no. 2182012), the National Natural Science Foundation (no. 11179033), and Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds, Scientific Research Project of Beijing Educational Committee (KM202010028007).

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Authors and Affiliations

  1. Department of Chemistry, Capital Normal University, Beijing, 100048, China

    Yuting Xue, Haotian Zhong, Bin Liu, Ruixue Zhao, Jun Ma, Zhengbo Chen, Kai Li & Xia Zuo

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  1. Yuting Xue
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  2. Haotian Zhong
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  3. Bin Liu
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  4. Ruixue Zhao
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  5. Jun Ma
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  6. Zhengbo Chen
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  7. Kai Li
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  8. Xia Zuo
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Correspondence to Zhengbo Chen or Xia Zuo.

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Xue, Y., Zhong, H., Liu, B. et al. Colorimetric sensing strategy for detection of cysteine, phenol cysteine, and phenol based on synergistic doping of multiple heteroatoms into sponge-like Fe/NPC nanozymes. Anal Bioanal Chem 414, 4217–4225 (2022). https://doi.org/10.1007/s00216-022-04074-8

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  • DOI: https://doi.org/10.1007/s00216-022-04074-8

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