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High-throughput method for the determination of nitroimidazoles in muscle samples by liquid chromatography coupled to mass spectrometry

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Abstract

We present a high-throughput confirmatory method for the analysis of 11 nitroimidazoles in muscle samples (metronidazole, MNZ; dimetridazole, DMZ; ronidazole, RNZ; tinidazole, TNZ; carnidazole, CRZ; secnidazole semihydrate, SCZ; ornidazole, ORZ; metronidazole-hydroxy, MNZ-OH; ipronidazole, IPZ; ipronidazole-hydroxy, IPZ-OH; and dimetridazole-hydroxy, HMMNI). Extraction with acetonitrile is efficient and simple, with the majority of recoveries ranging between 65 and 101 %, and without the need for clean-up of the extracts. The chromatographic analysis is performed using UPLC-QqQ-MS in the MRM mode, with an electrospray source operated in positive mode. Total chromatographic analysis time is 12 min. This method has been fully validated in muscle according to Decision 657/2002/CE guidelines, and the CCα values obtained were in the range of 0.04–0.4 μg·kg−1. The method is currently used for the analysis of muscle samples and has been tested in other matrices of animal origin, such as kidney, retina and egg, with excellent results.

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Authors and Affiliations

  1. Laboratori de l’Agència de Salut Pública de Barcelona, Avinguda Drassanes 13-15, 08001, Barcelona, Spain

    A. Rúbies, G. Sans, P. Kumar & F. Centrich

  2. Department of Analytical Chemistry, University of Barcelona, Martí Franquès 1-11, 08028, Barcelona, Spain

    G. Sans, P. Kumar, M. Granados & R. Companyó

  3. CIBER of Epidemiology and Public Health (CIBERESP), Madrid, Spain

    A. Rúbies & F. Centrich

Authors
  1. A. Rúbies
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  2. G. Sans
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  3. P. Kumar
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  4. M. Granados
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  5. R. Companyó
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  6. F. Centrich
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Correspondence to A. Rúbies.

Additional information

Published in the topical collection on Hormone and Veterinary Drug Residue Analysis with guest editors Siska Croubels, Els Daeseleire, Sarah De Saeger, Peter Van Eenoo, and Lynn Vanhaecke.

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Rúbies, A., Sans, G., Kumar, P. et al. High-throughput method for the determination of nitroimidazoles in muscle samples by liquid chromatography coupled to mass spectrometry. Anal Bioanal Chem 407, 4411–4421 (2015). https://doi.org/10.1007/s00216-014-8436-x

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  • DOI: https://doi.org/10.1007/s00216-014-8436-x

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