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Quantification of Piperazine in Chicken Muscle by Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

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Abstract

An ultra-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (UPLC-ESI/MS/MS) method was developed for the detection of piperazine in chicken muscle. Following extraction and purification, the chicken muscle extract was injected into the UPLC system and analyzed. Piperazine detection was performed on a triple-quadrupole mass spectrometer with the ESI interface operating in positive mode. The most sensitive mass transition from the precursor ion to the product ion was 87.1 → 44.1 for piperazine. The coefficient (R 2) of piperazine was 0.9995 in the range of 1–200 μg/kg. The recovery and relative standard deviation (RSD) ranged from 102.93 to 111.46% and from 4.57 to 5.28% at 50.0, 100.0 and 200.0 μg/kg adding levels, respectively. Limits of detection (LODs) and limits of quantification (LOQs) were 0.4 and 1.0 μg/kg, respectively. This method was fully validated based on its specificity, sensitivity, linearity, precision, accuracy, matrix effect, and stability results. Compared to other researches, this new method not only omitted the derivative process, which simplify the pre-processing, but also proved a better sensitivity based on its low LOD and LOQ values.

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Acknowledgements

This work was Supported by the China Agriculture Research System (CARS-42-G23), the National Natural Science Foundation of China (31302009), the National Science and Technology Pillar Program during the Twelfth Five-year Plan Period of China (2014BAD13B02), the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, and the New Century Talent Project of Yangzhou University.

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Authors and Affiliations

  1. College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China

    Kaizhou Xie, Ya’nan Liu, Lirui Sun, Qiang Gao, Bo Wang, Yangyang Zhang, Genxi Zhang, Guojun Dai & Jinyu Wang

  2. Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, 225009, China

    Kaizhou Xie, Ya’nan Liu, Lirui Sun, Qiang Gao, Bo Wang, Yangyang Zhang, Genxi Zhang, Guojun Dai & Jinyu Wang

  3. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, China

    Kaizhou Xie, Ya’nan Liu, Lirui Sun, Qiang Gao, Bo Wang, Yangyang Zhang, Genxi Zhang, Guojun Dai & Jinyu Wang

  4. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Maoda Pang, Xing Xie & Ran Wang

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  1. Kaizhou Xie
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  2. Ya’nan Liu
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  3. Lirui Sun
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  5. Xing Xie
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  7. Bo Wang
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  9. Ran Wang
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  10. Genxi Zhang
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  11. Guojun Dai
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Correspondence to Kaizhou Xie.

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Conflict of Interest

Kaizhou Xie declares that he has no conflict of interest. Ya’nan Liu declares that he has no conflict of interest. Lirui Sun declares that he has no conflict of interest. Maoda Pang declares that he has no conflict of interest. Xing Xie declares that he has no conflict of interest. Qiang Gao declares that he has no conflict of interest. Bo Wang declares that he has no conflict of interest. Yangyang Zhang declares that he has no conflict of interest. Ran Wang declares that he has no conflict of interest. Genxi Zhang declares that he has no conflict of interest. Guojun Dai declares that he has no conflict of interest. Jinyu Wang declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Xie, K., Liu, Y., Sun, L. et al. Quantification of Piperazine in Chicken Muscle by Ultra-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry. Food Anal. Methods 10, 1736–1744 (2017). https://doi.org/10.1007/s12161-016-0717-x

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