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Simultaneous Determination of Aminoglycoside Residues in Food Animal Muscles by Mixed-Mode Liquid Chromatography-Tandem Mass Spectrometry

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Abstract

A sensitive, selective, and reproducible method was developed for determining nine aminoglycoside antimicrobial residues in swine, cattle, mutton, chicken, and fish muscles by liquid chromatography-tandem mass spectrometry. The analytes were extracted with phosphate buffer solution and purified by a mixed-mode strong cation exchange solid-phase extraction cartridge. Using acetonitrile-1% formic acid solution as a mobile phase system, avoiding the use of the ion pair reagents, the target components retained well and effectively separated on the mixed-mode liquid chromatographic Obelisc R column. It was suggested that the aminoglycoside’s retention time increased with the increase of the number of amino groups in its molecule. The matrix-matched calibration curves for analytes were good linearity within the range of 5–500 μg L−1. The CCαs of analytes were between 2 and 10 μg kg−1, and CCβs were between 6 and 25 μg kg−1. In the spiked levels of 25, 50, and 100 μg kg−1, the average recoveries of analytes were in the range of 65–110% except neomycin of 60–75%, with relative standard deviation below 20%.

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Funding

The authors are grateful to the financial support from the National Science Foundation of China (31372476 and 31572562) and The National Key Research and Development Program of China (2016YFD0501305).

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Authors and Affiliations

  1. National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, 510642, China

    Jiufeng Li, Xuqin Song, Meiyu Zhang & Limin He

  2. Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China

    Erfen Li & Limin He

Authors
  1. Jiufeng Li
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  2. Xuqin Song
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  3. Meiyu Zhang
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  4. Erfen Li
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  5. Limin He
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Correspondence to Limin He.

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Conflict of Interest

Jiufeng Li declares that he has no conflict of interest. Xuqin Song declares that she has no conflict of interest. Meiyu Zhang declares that she has no conflict of interest. Erfen Li declares that she has no conflict of interest. Limin He declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of authors.

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Figure S1

The chemical structures of nine aminoglycoside antibiotics (GIF 109 kb)

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Li, J., Song, X., Zhang, M. et al. Simultaneous Determination of Aminoglycoside Residues in Food Animal Muscles by Mixed-Mode Liquid Chromatography-Tandem Mass Spectrometry. Food Anal. Methods 11, 1690–1700 (2018). https://doi.org/10.1007/s12161-018-1156-7

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  • DOI: https://doi.org/10.1007/s12161-018-1156-7

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