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Endophytes in the plant Huperzia serrata: fungal diversity and discovery of a new pentapeptide

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Abstract

Endophytic fungi are an underexploited resource of natural products and have a capacity to produce diverse classes of plant-derived secondary metabolites. Here, we investigated the diversity of endophytic fungi from Huperzia serrata and the potential for discovering novel fungal natural products. One hundred and fifty-five endophytic fungi isolates obtained from H. serrata, belonging to four classes Dothideomycetes (47.3 %), Sordariomycetes (36.8 %), Eurotiomycetes (10.6 %) and an undefined class (5.3 %, Mucoraceae), were grouped into nine genera based on morphological and molecular identification. Colletotrichum, Cladosporium, Sordariomycetes and Guignardia were the dominant genera, whereas the remaining genera were infrequent groups. To our knowledge, the fungal genera Mucor and Neurospora were first reported in Huperzia plant. Among these endophytic fungi isolates, strain B14, belonging to Penicillium oxalicum, gave a gray precipitate with Dragendorff’s reagent. A new pentapeptide was isolated from the culture of strain B14, and its chemical structure was elucidated on the basis of spectroscopic data from 1H NMR, 13C NMR and ESI–MS/MS. Taken together, H. serrata has a significant diversity of endophytic fungi that could be a rich resource for the discovery of new natural products.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program (“863” Program: 2012AA02A701), the National Basic Research Program of China (973 Program, Grant No. 2012CB721104), the National Natural Science Foundation of China (Grants No. 31170101 and 31100073), and the Knowledge Innovation Program of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Grant No. 2014KIP104), Shanghai Natural Science Foundation (Grant No.12ZR1435700).

Conflict of interest

The authors declare that they have no competing interest.

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Authors and Affiliations

  1. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China

    Zhi-Qiang Xiong, Ying-Ying Yang, Qiao-Xia Liu & Yong Wang

  2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Ying-Ying Yang

  3. Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China

    Cui-Cui Sun & Yu Jin

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  1. Zhi-Qiang Xiong
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  2. Ying-Ying Yang
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  5. Yu Jin
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  6. Yong Wang
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Correspondence to Yu Jin or Yong Wang.

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Communicated by Olaf Kniemeyer.

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Xiong, ZQ., Yang, YY., Liu, QX. et al. Endophytes in the plant Huperzia serrata: fungal diversity and discovery of a new pentapeptide. Arch Microbiol 197, 411–418 (2015). https://doi.org/10.1007/s00203-014-1072-1

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  • DOI: https://doi.org/10.1007/s00203-014-1072-1

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