Abstract
A strain LF70 endophytic fungus was isolated from the leaves of Huperzia serrata. The fungus was identified as Cladosporium cladosporioides LF70 according to its morphological characteristics and nuclear ribosomal DNA ITS sequence analysis. The strain could produce Huperzine A (HupA) identified through thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) with authentic HupA. The amount of HupA produced by this endophytic fungus was quantified to be 56.84 μg/L by HPLC, which was higher than that of other reported endophytic fungi, Acremonium sp., Blastomyces sp., and Botrytis sp. Acetylcholinesterase inhibition activity of HupA produced by strain LF70 was also similar to authentic HupA in vitro. Isolation of such a fungus may provide a promising alternative approach to producing HupA, which is used in treating Alzheimer’s disease and preventing further memory degeneration.
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Acknowledgments
This work was supported by Jiangxi Natural Science Foundation of China (Grant No. 2008GQN0075) and the Public Welfare Foundation of Key Lab of Protection and Utilization of Subtropic Plant Resources, Jiangxi province. We thank Prof. Lehua Zhang from Lushan botanical garden, China for helping with the collection and identification of the plant material. We also wish to thank the anonymous reviewers for constructive comments.
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Zhang, Z.B., Zeng, Q.G., Yan, R.M. et al. Endophytic fungus Cladosporium cladosporioides LF70 from Huperzia serrata produces Huperzine A. World J Microbiol Biotechnol 27, 479–486 (2011). https://doi.org/10.1007/s11274-010-0476-6
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DOI: https://doi.org/10.1007/s11274-010-0476-6