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Transferring a new Fusarium head blight resistance locus FhbRc1 from Roegneria ciliaris into wheat by developing alien translocation lines

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Abstract

Key message

A new FHB resistance locus FhbRc1 was identified from the R. ciliaris chromosome 7Sc and transferred into common wheat by developing alien translocation lines.

Abstract

Fusarium head blight (FHB) caused by multiple Fusarium species is a globally destructive disease of common wheat. Exploring and utilization of resources with FHB resistance are the most effective and environmentally beneficial approach for the disease control. Roegneria ciliaris (Trin.) Nevski (2n = 4x = 28, ScScYcYc), a tetraploid wheat wild relative, possesses high resistance to FHB. In the previous study, a complete set of wheat-R. ciliaris disomic addition (DA) lines were evaluated for FHB resistance. DA7Sc had stable FHB resistance, which was confirmed to be derived from alien chromosome 7Sc. We tentatively designated the resistant locus as FhbRc1. For better utilization of the resistance in wheat breeding, we developed translocations by inducing chromosome structural aberrations using iron irradiation and the homologous pairing gene mutant ph1b. Totally, 26 plants having various 7Sc structural aberrations were identified. By marker analysis, a cytological map of 7Sc was constructed and 7Sc was dissected into 16 cytological bins. Seven alien chromosome aberration lines, which all had the bin 7Sc-1 on the long arm of 7Sc, showed enhanced FHB resistance. Thus, FhbRc1 was mapped to the distal region of 7ScL. A homozygous translocation line T4BS·4BL-7ScL (NAURC001) was developed. It showed improved FHB resistance, while had no obvious genetic linkage drag for the tested agronomic traits compared with the recurrent parent Alondra’s. When transferring the FhbRc1 into three different wheat cultivars, the derived progenies having the translocated chromosome 4BS·4BL-7ScL all showed improved FHB resistance. This revealed the potential value of the translocation line in wheat breeding for FHB resistance.

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Data availability

Phenotype data generated or analyzed during this study are included in this published article (and its supplementary files). Genotypic data are available from the corresponding author on reasonable request.

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Acknowledgements

We are grateful to Professor Robert McIntosh from University of Sydney, Australia, for revising the manuscript and insight comment.

Funding

This research was supported by the National Key Research and Development Program (2018YFE0107700), the Fundamental Research Funds for the Central Universities (KYZZ2022003), the National Key Research and Development Program (2020YFE0202900), International Cooperation and Exchange of the National Natural Science Foundation of China (31661143005), Jiangsu Provincial Key Research and Development Program (BE2022346), Seed Industry Revitalization Project of Jiangsu Province (JBGS(2021)006, JBGS(2021)013 and JBGS(2021)047), the Joint Research of Improved Wheat Variety of Anhui, the Key Research and Development Major Project of Ningxia Autonomous Region (No. 2019BBF02022-04), the Jiangsu Agricultural Technology System (JATS) (JATS[2020]411, JATS[2021]463 and JATS[2022]464).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, Jiangsu, 210095, China

    Rongrong Song, Yifan Cheng, Mingxing Wen, Xinying Song, Tong Wang, Mengshuang Xia, Haojie Sun, Menghao Cheng, Huimin Cui, Chunxia Yuan, Xiaoxue Liu, Zongkuan Wang, Li Sun, Haiyan Wang, Jin Xiao & Xiue Wang

  2. Zhenjiang Institute of Agricultural Science, Jurong, Jiangsu, 212400, China

    Mingxing Wen

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  1. Rongrong Song
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  2. Yifan Cheng
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  3. Mingxing Wen
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  4. Xinying Song
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  5. Tong Wang
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  8. Menghao Cheng
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  16. Xiue Wang
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Contributions

RRS developed and characterized the chromosomal aberrations using molecular markers and GISH/FISH, conducted FHB resistance evaluation, conducted the field investigation of agronomic traits and wrote the manuscript. XYS developed and evaluated the FHB resistance of the original DALs. YFC, MXW, MHC and HMC conducted FHB resistance evaluation and field managements. HJS, TW and MSX conducted the GISH/FISH analysis. CXY, XXL, ZKW and LS conducted field management and seed propagation in the growth chamber. HYW supervised the development of alien chromosomal lines and revised the manuscript. JX conducted the FHB resistance evaluation, discussed the results and wrote the manuscript. XEW designed and supervised the research, discussed the results, coordinated the group work and wrote the manuscript. All authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Jin Xiao or Xiue Wang.

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Communicated by Thomas Miedaner.

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Song, R., Cheng, Y., Wen, M. et al. Transferring a new Fusarium head blight resistance locus FhbRc1 from Roegneria ciliaris into wheat by developing alien translocation lines. Theor Appl Genet 136, 36 (2023). https://doi.org/10.1007/s00122-023-04278-1

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