Abstract
Key message
Discovery and mapping of a susceptibility factor located on the short arm of wheat chromosome 7A whose deletion makes plants resistant to Fusarium head blight.
Abstract
Fusarium head blight (FHB) disease of wheat caused by Fusarium spp. deteriorates both quantity and quality of the crop. Manipulation of susceptibility factors, the plant genes facilitating disease development, offers a novel and alternative strategy for enhancing FHB resistance in plants. In this study, a major effect susceptibility gene for FHB was identified on the short arm of chromosome 7A (7AS). Nullisomic–tetrasomic lines for homoeologous group-7 of wheat revealed dosage effect of the gene, with tetrasomic 7A being more susceptible than control Chinese Spring wheat, qualifying it as a genuine susceptibility factor. Five chromosome 7A inter-varietal substitution lines and a tetraploid Triticum dicoccoides 7A substitution line showed similar susceptibility as that of Chinese Spring, indicating toward the commonality of the susceptibility factor among these diverse genotypes. The susceptibility factor was named as Sf-Fhb-7AS and mapped on chromosome 7AS to a 48.5–50.5 Mb peri-centromeric region between del7AS-3 and del7AS-8. Our results showed that deletion of Sf-Fhb-7AS imparts 50–60% type 2 FHB resistance and its manipulation can be used to enhance resistance against FHB in wheat.
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Acknowledgements
Authors are thankful to US Wheat Barley and Scab Initiative (Award# 59-0206-0-177, 59-0200-6-018), National Science Foundation (Award# 1943155) and USDA NIFA (Award# 2020-67013-32558 and 2020-67013-31460) for financial support.
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BC performed the experiments, recorded data, conducted statistical analyses and co-wrote the manuscript with NR. VT, BSG and NR developed the idea, BSG provided the genetic stocks for conducting the study, YD performed DON content measurement, and NR designed the experiments and arranged funding resources for conducting the experiments. All co-authors provided their inputs in the manuscript.
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Chhabra, B., Tiwari, V., Gill, B.S. et al. Discovery of a susceptibility factor for Fusarium head blight on chromosome 7A of wheat. Theor Appl Genet 134, 2273–2289 (2021). https://doi.org/10.1007/s00122-021-03825-y
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DOI: https://doi.org/10.1007/s00122-021-03825-y