Abstract
Hairy root culture (HRC) is a promising biotechnological tool for the production of plant secondary metabolites under in vitro condition. This has been harnessed for the production of several molecules of medicinal and commercial importance. The hairy root phenotype appears at the site of infection of plants by Rhizobium rhizogenes which possess characteristics like fast growth even without hormone in the media, high genetic stability, geotropism, profuse lateral branching, differentiated tissue type, and high productivity. The content of secondary metabolites in these hairy roots is usually comparable to that of the fully grown plants. But, the major bottleneck for commercial exploitation of HRC is the developing protocol for scaling up production in bioreactors, as easy handling of interconnected hairy roots that are unevenly distributed in the vessel, is difficult. This chapter is focused on HRC with latest information available on basic method of induction of hairy roots to its uses in production of low volume and up-scaling in bioreactors, for commercial viability of HRC-based technology.
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Singh, R.S., Singh, P., Kumari, R., Kumar, S. (2023). Hairy Root Cultures for Secondary Metabolite Production. In: Singh, R., Kumar, N. (eds) Genetic Manipulation of Secondary Metabolites in Medicinal Plant. Interdisciplinary Biotechnological Advances. Springer, Singapore. https://doi.org/10.1007/978-981-99-4939-7_9
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