Abstract
Artemisinin production by hairy roots of Artemisia annua L. was increased 6-fold to 1.8 μg mg−1 dry wt over 6 days by adding 150 mg chitosan l−1. The increase was dose-dependent. Similar treatment of hairy roots with methyl jasmonate (0.2 mM) or yeast extract (2 mg ml−1) increased artemisinin production to 1.5 and 0.9 μg mg−1 dry wt, respectively.
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Acknowledgements
This work was supported by a grant from the National Center for Genetic and Biotechnology (BIOTEC), Thailand and Asian Core Program (Medicinal Plant Breeding Division) under the Japan Society for the Promotion of Science (JSPS).
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Putalun, W., Luealon, W., De-Eknamkul, W. et al. Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L.. Biotechnol Lett 29, 1143–1146 (2007). https://doi.org/10.1007/s10529-007-9368-8
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DOI: https://doi.org/10.1007/s10529-007-9368-8