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Methods and Instrumentation in Mass Spectrometry for the Differentiation of Closely Related Microorganisms

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Applications of Mass Spectrometry in Microbiology

Abstract

While mass spectrometry (MS) is now an accepted tool for the routine analysis of microorganisms, new applications using MS are now tackling more complex problems in microbiology. The successful application of MS for the analysis of microorganisms involves a concerted selection of hardware along with the development of methodology. This chapter will focus on this interplay between MS instrumentation and methodology for the analysis of closely related bacteria at the strain level and antibiotic-resistant/susceptible bacteria. An overview of the MS instrumentation will be presented in the context of microorganism detection and identification with selected examples from the current scientific literature.

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Authors and Affiliations

  1. Department of Chemistry, Biodetection and Mass Spectrometry Laboratory, University of Wyoming, 1000 E. University Ave (Department 3838), 82071, Laramie, WY, USA

    Franco Basile PhD & Rudolph K. Mignon

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  1. Franco Basile PhD
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  2. Rudolph K. Mignon
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Correspondence to Franco Basile PhD .

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  1. Johns Hopkins University, Applied Physics Laboratory, Laurel, Maryland, USA

    Plamen Demirev

  2. Arizona State University, School of Math and Natural Sciences, Phoenix, Arizona, USA

    Todd R. Sandrin

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Basile, F., Mignon, R. (2016). Methods and Instrumentation in Mass Spectrometry for the Differentiation of Closely Related Microorganisms. In: Demirev, P., Sandrin, T. (eds) Applications of Mass Spectrometry in Microbiology. Springer, Cham. https://doi.org/10.1007/978-3-319-26070-9_2

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