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Evaluation of two matrix-assisted laser desorption ionization–time of flight mass spectrometry systems for identification of viridans group streptococci

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Abstract

In this study, the performances of two matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) systems, MALDI Biotyper (Bruker Daltonics) and VITEK MS (bioMérieux), were evaluated in the identification of viridans group streptococci. Two collections of isolates were tested with both methods. From a panel of type collection strains (n = 54), MALDI Biotyper gave correct species-level identification for 51/54 (94 %) strains and 37/54 (69 %) strains for the VITEK MS in vitro diagnostic (IVD) method. Additionally, a collection of blood cultures isolates which had been characterized earlier with partial sequencing of 16S rRNA (n = 97) was analyzed. MALDI Biotyper classified 89 % and VITEK MS 93 % of these correctly to the group level. Comparison of species-level identification from the blood culture collection was possible for 36 strains. MALDI Biotyper identified 75 % and VITEK MS 97 % of these strains consistently. Among the clinical isolates, MALDI Biotyper misidentified 36 strains as Streptococcus pneumoniae. Nevertheless, our results suggest that the current MALDI-TOF methods are a good alternative for the identification of viridans streptococci and do perform as well as or better than commercial phenotypical methods.

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Acknowledgments

We thank Piritta Peri, Saija Vahanne, Saana Viertomanner, Laura Juska, and Janne Tarhanen for their skillful technical assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Clinical Microbiology, Päijät-Häme Social and Health Care Group, Center of Medical Services, Keskussairaalankatu 7, 15850, Lahti, Finland

    P. Kärpänoja & H. Sarkkinen

  2. Clinical Microbiology Laboratory, Turku University Hospital, Turku, Finland

    I. Harju & K. Rantakokko-Jalava

  3. Antimicrobial Resistance Unit, National Institute for Health and Welfare, Turku, Finland

    M. Haanperä

Authors
  1. P. Kärpänoja
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  2. I. Harju
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  3. K. Rantakokko-Jalava
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  4. M. Haanperä
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  5. H. Sarkkinen
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Corresponding author

Correspondence to P. Kärpänoja.

Additional information

Pauliina Kärpänoja and Inka Harju contributed equally in this study.

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Kärpänoja, P., Harju, I., Rantakokko-Jalava, K. et al. Evaluation of two matrix-assisted laser desorption ionization–time of flight mass spectrometry systems for identification of viridans group streptococci. Eur J Clin Microbiol Infect Dis 33, 779–788 (2014). https://doi.org/10.1007/s10096-013-2012-8

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  • DOI: https://doi.org/10.1007/s10096-013-2012-8

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