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Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z ≈ 29,000 peak, which was confirmed as β-lactamase by in-gel digestion followed by peptide mass fingerprinting. Rapid MALDI-TOF MS detection of antibiotic-resistance could fulfill an important clinical need, providing critical phenotypic information beyond genus–species identification.

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Authors and Affiliations

  1. Analytical Chemistry Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA

    Johanna E. Camara & Faith A. Hays

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  1. Johanna E. Camara
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  2. Faith A. Hays
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Correspondence to Johanna E. Camara.

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Camara, J.E., Hays, F.A. Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Bioanal Chem 389, 1633–1638 (2007). https://doi.org/10.1007/s00216-007-1558-7

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  • DOI: https://doi.org/10.1007/s00216-007-1558-7

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