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Gene Editing in Sorghum Through Agrobacterium

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Sorghum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1931))

Abstract

The application of CRISPR/Cas to introduce targeted genomic edits is powering research and discovery across the genetic frontier. Applying CRISPR/Cas in sorghum can facilitate the study of gene function and unlock our understanding of this robust crop that serves as a staple for some of the most food insecure regions on the planet. When paired with recent advances in sorghum tissue culture and Agrobacteria technology, CRISPR/Cas can be used to introduce desirable changes and natural genetic variations directly into agriculturally relevant sorghum lines facilitating product development. This chapter describes CRISPR/Cas gene editing and provides high-level strategies and expectations for applying this technology using Agrobacterium in sorghum.

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Acknowledgments

The author would like to acknowledge the Crop Genome Engineering, Vector Construction, Molecular Characterization, and Controlled Environment groups from Corteva Agriscience™, Agriculture Division of DowDuPont™ for support and Clara Alarcon and Todd J. Jones for comments on the manuscript. This work was funded by a subaward from the CSIRO under the Capturing Heterosis for Smallholder Farmers grant from BMGF. Corteva Agriscience™, Agriculture Division of DowDuPont™ provided funding and in-kind donations.

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Authors and Affiliations

  1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Jeffry D. Sander

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  1. Jeffry D. Sander
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Correspondence to Jeffry D. Sander .

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Editors and Affiliations

  1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Zuo-Yu Zhao

  2. UC-ANR Kearney Agricultural Research and Extension (KARE) Center, Parlier, CA, USA

    Jeff Dahlberg

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Sander, J.D. (2019). Gene Editing in Sorghum Through Agrobacterium. In: Zhao, ZY., Dahlberg, J. (eds) Sorghum. Methods in Molecular Biology, vol 1931. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9039-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9039-9_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9038-2

  • Online ISBN: 978-1-4939-9039-9

  • eBook Packages: Springer Protocols

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