Skip to main content
SpringerLink
Log in

Guide to Data Acquisition and Analysis

  • Chapter
Single-Channel Recording

Abstract

This chapter should provide a first guide to the acquisition and analysis of patch-clamp data Except for the sections on single-channel analysis, the procedures and considerations also hold for data obtained using other voltage-clamp methods. It is assumed that the reader is familiar with the standard methods and terminology of patch-clamp electrophysiology. Because many of the problems that arise during data analysis can be avoided by a proper design of the experiment, including data acquisition, we start by deriving some criteria that should be considered before actually starting to record data.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 299.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 379.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Armstrong, C. M., and Bezanilla, F., 1974, Charge movement associated with the opening and closing of the activation gates of the sodium channel, J. Gen. Physiol. 63: 533–552.

    Article  PubMed  CAS  Google Scholar 

  • Auerbach, A., 1993, A statistical analysis of acetylcholine receptor activation in Xenopus myocytes: Stepwise versus concerted models of gating, J. Physiol. 461: 339–378.

    PubMed  CAS  Google Scholar 

  • Bezanilla, F., 1985, A high capacity data recording device based on a digital audio processor and a video cassette recorder, Biophys. J. 47: 437–442.

    Article  PubMed  CAS  Google Scholar 

  • Blatz, A. L., and Magleby, K. L., 1986, Correcting single channel data for missed events, Biophys. J. 49: 967980.

    Google Scholar 

  • Blatz, A. L., and Magleby, K. L., 1989, Adjacent interval analysis distinguishes among gating mechanisms for the fast chloride channel from rat skeletal muscle, J. Physiol. 410: 561–585.

    PubMed  CAS  Google Scholar 

  • Chung, S. H., Moore, J. B., Xia, L. G., Premkumar, L. S., and Gage, P. W., 1990, Characterization of single channel currents using digital signal processing techniques based on hidden Markov models, Phil. Trans. R. Soc. Lond. B 329: 265–285.

    Article  CAS  Google Scholar 

  • Chung, S. H., Krishnamurthy, V., and Moore, J. B., 1991, Adaptive processing techniques based on hidden Markov models for characterizing very small channel currents buried in noise and deterministic interferences, Phil. Trans. R. Soc. Lond. B 334: 357–384.

    Article  CAS  Google Scholar 

  • Colquhoun, D. 1987, Practical analysis of single channel records, in: Microelectrode Techniques. The Plymouth Workshop Handbook ( N. B. Standen, P. T. A. Gray, and M. J. Whitaker, eds.), pp. 83–104, Company of Biologists Ltd., Cambridge.

    Google Scholar 

  • Colquhoun, D., and Sakmann, B., 1985, Fast events in single-channel currents activated by acetylcholine and its analogues at the frog muscular end-plate, J. Physiol. 369: 501–557.

    PubMed  CAS  Google Scholar 

  • Crouzy, S. C., and Sigworth, F. J., 1990, Yet another approach to the dwell-time omission problem of single-channel analysis, Biophys. J. 58: 731–743.

    Article  PubMed  CAS  Google Scholar 

  • DeFelice, L. J., 1981, Introduction to Membrane Noise, Plenum Press, New York.

    Book  Google Scholar 

  • Dempster, J., 1993, Computer Analysis of Electrophysiological Signals, Academic Press, London.

    Google Scholar 

  • Fredkin, D. R., and Rice, J. A., 1992, Maximum likelihood estimation and identification directly from single-channel recordings, Proc. R. Soc. Lond. B 249: 125–132.

    Article  CAS  Google Scholar 

  • French, R. J., and Wonderlin, W. F., 1992, Software for acquisition and analysis of ion channel data: Choices, tasks, and strategies, in: Ion Channels, Methods in Enzymology, Vol. 207 ( B. Rudy and L. E. Iverson, eds.), pp. 711–728, Academic Press, San Diego.

    Google Scholar 

  • Hawkes, A. G., Jalali, A., and Colquhoun, D., 1991, The distribution of open and shut times in a single channel record when brief events cannot be detected, Phil. Trans. R. Soc. Lond. A 332: 511–538.

    Google Scholar 

  • Heinemann, S. H., and Conti, E, 1992, Non-stationary noise analysis and its application to patch clamp recordings, in: Ion Channels, Methods in Enzymology, Vol. 207 ( B. Rudy and L. E. Iverson, eds.), pp. 131–148, Academic Press, San Diego.

    Google Scholar 

  • Heinemann, S. H., and Sigworth, F. J., 1988, Open channel noise. IV. Estimation of rapid kinetics of formamide block in gramicidin A channels, Biophys. J. 54: 757–764.

    Article  PubMed  CAS  Google Scholar 

  • Heinemann, S. H., and Sigworth, F. J., 1989, Estimation of Na` dwell time in the gramicidin A channel. Na ions as blockers of H. currents, Biochim. Biophys. Acta 987: 8–14.

    Article  PubMed  CAS  Google Scholar 

  • Heinemann, S. H., and Sigworth, F. J., 1989, Estimation of Na* dwell time in the gramicidin A channel. Na ions as blockers of H. currents, Biochim. Biophys. Acta 987: 8–14.

    Article  PubMed  CAS  Google Scholar 

  • Heinemann, S. H., and Sigworth, F. J., 1990, Open channel noise. V. A fluctuating barrier to ion entry in gramicidin A channels, Biophys. J. 57: 499–514.

    Article  PubMed  CAS  Google Scholar 

  • Heinemann, S. H., and Sigworth, F. J., 1991, Open channel noise. VI. Analysis of amplitude histograms to determine rapid kinetic parameters, Biophys. J. 60: 577–587.

    Article  PubMed  CAS  Google Scholar 

  • Heinemann, S. H., Conti, F., and Stühmer, W., 1992, Recording of gating currents from Xenopus oocytes and gating noise analysis, in: Ion Channels, Methods in Enzymology, Vol. 207 ( B. Rudy and L. E. Iverson, eds.), pp. 353–368, Academic Press, San Diego.

    Google Scholar 

  • Hess, P., Lansman, J. B., and Tsien, R. W., 1984, Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists, Nature 311: 538–544.

    Article  PubMed  CAS  Google Scholar 

  • Hodgkin, A. L., and Huxley, A. H., 1952, A quantitative description of membrane current and its application to conduction and excitation in nerve, J. Physiol. 117: 500–544.

    PubMed  CAS  Google Scholar 

  • Hoshi, T., Zagotta, W. N., and Aldrich, R. W., 1990, Biophysical and molecular mechanisms of Shaker potassium channel inactivation, Science 250: 533–538.

    Article  PubMed  CAS  Google Scholar 

  • Jackson, M. B., 1992, Stationary single-channel analysis, in: Ion Channels, Methods in Enzymology, Vol. 207 ( B. Rudy and L. E. Iverson, eds.), pp. 729–746, Academic Press, San Diego.

    Google Scholar 

  • Kirlin, R. L., and Moghaddamjoo, A., 1986, A robust running window detector and estimator for step signals in contaminated Gaussian noise, IEEE Trans. Acoust. Speech Signal Process. 34: 816–823.

    Article  Google Scholar 

  • Magleby, K. L., 1992, Preventing aritfacts and reducing errors in single-channel analysis, in: Ion Channels, Methods in Enzymology, Vol. 207 ( B. Rudy and L. E. Iverson, eds.), pp. 763–791, Academic Press, San Diego.

    Google Scholar 

  • Magleby, K. L., and Weiss, D. S., 1990, Estimating kinetic parameters for single channels for simulation. A general method that resolves the missing event problem and accounts for noise, Biophys. J. 58: 1411–1426.

    Article  PubMed  CAS  Google Scholar 

  • McManus, O. B., Blatz, A. L., and Magleby, K. L., 1987, Sampling, log binning, fitting and plotting durations of open and shut intervals from single channels and the effect of noise, Pflügers Arch. 410: 530–553.

    Article  PubMed  CAS  Google Scholar 

  • Moghaddamjoo, A., Levis, R. A., and Eisenberg, R. S., 1988, Automatic detection of channel currents, Biophys. J. 53: 153a.

    Google Scholar 

  • Neher, E., and Stevens, C. F., 1977, Conductance fluctuations and ionic pores in membranes, Ann. Rev. Biophys. Bioeng. 6: 345–381.

    Article  CAS  Google Scholar 

  • Nyquist, H., 1928, Certain topics in telegraph transmission theory, Trans AIEE 47.

    Google Scholar 

  • Pastushenko, V. P., and Schindler, H., 1993, Statistical filtering of single channel ion channel records, Acta Pharm. 43: 7–13.

    CAS  Google Scholar 

  • Patlak, J. B., 1988, Sodium channel subconductance levels measured with a new variance—mean analysis, J. Gen. Physiol. 92: 413–430.

    Article  PubMed  CAS  Google Scholar 

  • Patlak, J. B., 1993, Measuring kinetics of complex single ion channel data using mean-variance histograms, Biophys. J. 65: 29–42.

    Article  PubMed  CAS  Google Scholar 

  • Roux, B., and Sauve, R., 1985, A general solution to the time interval omission problem applied to single channel analysis, Biophys. J. 48: 149–158.

    Article  PubMed  CAS  Google Scholar 

  • Sachs, F., Neil, J., and Barkakati, N., 1982, The automated analysis of data from single ionic channels, Pflügers Arch. 395: 331–340.

    Article  PubMed  CAS  Google Scholar 

  • Sherman-Gold, R. (ed.), 1993, The Axon Guide, Axon Instruments, Inc., Foster City, CA.

    Google Scholar 

  • Sigworth, F. J., 1980, The variance of sodium current fluctuations at the node of Ranvier, J. Physiol. 307: 97129.

    Google Scholar 

  • Sigworth, F. J., 1985, Open channel noise. I. Noise in acetylcholine receptor currents suggests conformational fluctuations, Biophys. J. 47: 709–720.

    Article  PubMed  CAS  Google Scholar 

  • Sigworth, E J., and Sine, S. M., 1987, Data transformations for improved display and fitting of single-channel dwell time histograms, Biophys. J. 48: 149–158.

    Google Scholar 

  • Sigworth, E. J., and Zhou, J., 1992, Analysis of nonstationary single-channel currents, in: Ion Channels, Methods in Enzymology, Vol. 207 ( B. Rudy and L. E. Iverson, eds.), pp. 746–762, Academic Press, San Diego.

    Google Scholar 

  • Yellen, G., 1984, Ionic permeation and blockade in Caactivated Kchannels of bovine chromaffin cells, J. Gen. Physiol. 84: 157–186.

    Article  PubMed  CAS  Google Scholar 

  • Zhou, J.,Potts, J. F., Trimmer, J. S., Agnew, W. S., and Sigworth, F. J., 1991, Multiple gating modes and the effect of modulating factors on the µI sodium channel, Neuron 7:775–785.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular and Cellular Biophysics, “Max Planck Society, Research Unit”, D-07747, Jena, Germany

    Stefan H. Heinemann

Authors
  1. Stefan H. Heinemann
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Cell Physiology, Max-Planck-Institute for Medical Research, D-69120, Heidelberg, Germany

    Bert Sakmann

  2. Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg, D-37077, Göttingen, Germany

    Erwin Neher

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer Science+Business Media New York

About this chapter

Cite this chapter

Heinemann, S.H. (1995). Guide to Data Acquisition and Analysis. In: Sakmann, B., Neher, E. (eds) Single-Channel Recording. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-1229-9_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4419-1229-9_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4419-1230-5

  • Online ISBN: 978-1-4419-1229-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation