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Shrink-Induced Biomimetic Wrinkled Substrates for Functional Cardiac Cell Alignment and Culture

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Cardiac Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1181))

Abstract

The anisotropic alignment of cardiomyocytes in native myocardium tissue is a functional feature that is absent in traditional in vitro cardiac cell culture. Microenvironmental factors cue structural organization of the myocardium, which promotes the mechanical contractile properties and electrophysiological patterns seen in mature cardiomyocytes. Current nano- and microfabrication techniques, such as photolithography, generate simplified cell culture topographies that are not truly representative of the multifaceted and multi-scale fibrils of the cardiac extracellular matrix. In addition, such technologies are costly and require a clean room for fabrication. This chapter offers an easy, fast, robust, and inexpensive fabrication of biomimetic multi-scale wrinkled surfaces through the process of plasma treating and shrinking prestressed thermoplastic. Additionally, this chapter includes techniques for culturing stem cells and their cardiac derivatives on these substrates. Importantly, this wrinkled cell culture platform is compatible with both fluorescence and bright-field imaging; real-time physiological monitoring of CM action potential propagation and contraction properties can elucidate cardiotoxicity drug effects.

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Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, University of California, 5200 Engineering Hall Irvine, Irvine, CA, USA

    Nicole Mendoza, Eugene Lee & Michelle Khine Ph.D.

  2. Department of Biological Sciences, University of California, Irvine, CA, USA

    Roger Tu

  3. Department of Chemical Engineering and Materials Science, University of California, 5200 Engineering Hall Irvine, Irvine, CA, USA

    Aaron Chen & Michelle Khine Ph.D.

Authors
  1. Nicole Mendoza
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  2. Roger Tu
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  3. Aaron Chen
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  4. Eugene Lee
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  5. Michelle Khine Ph.D.
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Corresponding author

Correspondence to Michelle Khine Ph.D. .

Editor information

Editors and Affiliations

  1. Department Chemical Engineering&Applied Chemistry, University of Toronto, Toronto, Ontario, Canada

    Milica Radisic

  2. Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA

    Lauren D. Black III

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Mendoza, N., Tu, R., Chen, A., Lee, E., Khine, M. (2014). Shrink-Induced Biomimetic Wrinkled Substrates for Functional Cardiac Cell Alignment and Culture. In: Radisic, M., Black III, L. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 1181. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1047-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-1047-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1046-5

  • Online ISBN: 978-1-4939-1047-2

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