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Genotype Phenotype Coupling

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Stefan Zielonka

    1. Protein Engineering and Antibody Technologies (PEAT), Merck Healthcare KGaA, Darmstadt, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Simon Krah

    1. Protein Engineering and Antibody Technologies (PEAT), Merck Healthcare KGaA, Darmstadt, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2681)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Quantitative Determination of Staphylococcus aureus Using Aptamer-Based Recognition and DNA Amplification Machinery

    • Nandi Zhou, Rongfeng Cai
    Pages 1-18

  3. Construction of Synthetic VHH Libraries in Ribosome Display Format

    • Audrey Guilbaud, Frédéric Pecorari
    Pages 19-31

  4. Isolation of Adhirons Specific for Plant Protoplast Membrane Biomarkers Is Simplified by Phagemid Design

    • Claudia D’Ercole, Ario de Marco
    Pages 33-45

  5. Facile One-Step Generation of Camelid VHH and Avian scFv Libraries for Phage Display by Golden Gate Cloning

    • Christina Bauer, Elke Ciesielski, Lukas Pekar, Simon Krah, Lars Toleikis, Stefan Zielonka et al.
    Pages 47-60

  6. Identification of New Antibodies Targeting Tumor Cell Surface Antigens by Phage Display

    • Steffen Krohn, Matthias Peipp, Katja Klausz
    Pages 61-82

  7. Phage Display of Bovine Ultralong CDRH3

    • Callum Joyce, Louise Speight, Alastair D. G. Lawson, Anthony Scott-Tucker, Alex Macpherson
    Pages 83-97

  8. Bacterial Cell Display for Selection of Affibody Molecules

    • Charles Dahlsson Leitao, Stefan StÃ¥hl, John Löfblom
    Pages 99-112

  9. Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

    • Paul Arras, Jasmin Zimmermann, Britta Lipinski, Desislava Yanakieva, Daniel Klewinghaus, Simon Krah et al.
    Pages 113-129

  10. Selection of High-Affinity Heterodimeric Antigen-Binding Fc Fragments from a Large Yeast Display Library

    • Filippo Benedetti, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Rüker, Gordana Wozniak-Knopp
    Pages 131-159

  11. A Two-Step Golden Gate Cloning Procedure for the Generation of Natively Paired YSD Fab Libraries

    • Lena Vollmer, Simon Krah, Stefan Zielonka, Desislava Yanakieva
    Pages 161-173

  12. Single-Cell B-Cell Sequencing to Generate Natively Paired scFab Yeast Surface Display Libraries

    • Nathaniel Pascual, Theodore Belecciu, Sam Schmidt, Athar Nakisa, Xuefei Huang, Daniel Woldring
    Pages 175-212

  13. One-Pot Droplet RT-OE-PCR for the Generation of Natively Paired Antibody Immune Libraries

    • Desislava Yanakieva, Lena Vollmer, Satyendra Kumar, Stefan Becker, Lars Toleikis, Lukas Pekar et al.
    Pages 213-229

  14. Affinity Maturation of the Natural Ligand (B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast Surface Display

    • Stefan Zielonka, Simon Krah, Paul Arras, Britta Lipinski, Jasmin Zimmermann, Ammelie Svea Boje et al.
    Pages 231-248

  15. Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening

    • Jorge A. Lerma Romero, Harald Kolmar
    Pages 249-274

  16. Tyrosine Phosphorylation Screening on the Yeast Surface by Magnetic Bead Selection and FACS

    • Jose Ezagui, Lawrence A. Stern
    Pages 275-290

  17. Bulk Reformatting of Antibody Fragments Displayed on the Surface of Yeast Cells to Final IgG Format for Mammalian Production

    • Stefania C. Carrara, Jan P. Bogen, David Fiebig, Julius Grzeschik, Björn Hock, Harald Kolmar
    Pages 291-311

  18. Antibody-Secreting Cell Isolation from Different Species for Microfluidic Antibody Hit Discovery

    • Ramona Gaa, Qingyong Ji, Achim Doerner
    Pages 313-325

  19. Efficient Microfluidic Downstream Processes for Rapid Antibody Hit Confirmation

    • Ramona Gaa, Hannah Melina Mayer, Daniela Noack, Achim Doerner
    Pages 327-341

  20. Cell Line Development Using Targeted Gene Integration into MAR-Rich Landing Pads for Stable Expression of Transgenes

    • Claudia Oliviero, Steffen C. Hinz, Julius Grzeschik, Björn Hock, Harald Kolmar, Gerrit Hagens
    Pages 343-359
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Keywords

About this book

This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.

Editors and Affiliations

  • Protein Engineering and Antibody Technologies (PEAT), Merck Healthcare KGaA, Darmstadt, Germany

    Stefan Zielonka, Simon Krah

Bibliographic Information

  • Book Title: Genotype Phenotype Coupling

  • Book Subtitle: Methods and Protocols

  • Editors: Stefan Zielonka, Simon Krah

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3279-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3278-9Published: 06 July 2023

  • Softcover ISBN: 978-1-0716-3281-9Due: 06 August 2023

  • eBook ISBN: 978-1-0716-3279-6Published: 05 July 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 408

  • Number of Illustrations: 10 b/w illustrations, 65 illustrations in colour

  • Topics: Biological Techniques, Genetics and Genomics, Biotechnology

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