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Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

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Mass Spectrometry of Glycoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2271))

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be regarded as a key tool to rapidly obtain molecular mass information of intact glycoproteins in glycoproteomic studies and quality control of recombinant biopharmaceuticals. However, MALDI-TOF MS of these glycosylated compounds is a tricky task due to its low ionization efficiency and fragmentation of labile groups such as sialic acids.

Here, we offer the reader a practical overview of the available methodologies for the confident analysis of intact glycoproteins with different glycosylation degree by MALDI-TOF MS. The three proposed methods fulfil the requirements of reproducibility and low extent of glycan fragmentation required to successfully analyze intact glycoproteins.

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Acknowledgments

This work was supported by a grant from the Spanish Ministry of Economy and Competitiveness (RTI2018-097411-B-I00) and the Cathedra UB Rector Francisco Buscarons Ubeda (Forensic Chemistry and Chemical Engineering). Montserrat Mancera-Arteu acknowledges the University of Barcelona for an ADR fellowship.

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Authors and Affiliations

  1. Department of Chemical Engineering and Analytical Chemistry, Institute for Research on Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain

    Estela Giménez, Montserrat Mancera-Arteu, Fernando Benavente & Victoria Sanz-Nebot

Authors
  1. Estela Giménez
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  2. Montserrat Mancera-Arteu
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  3. Fernando Benavente
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  4. Victoria Sanz-Nebot
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Corresponding author

Correspondence to Estela Giménez .

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Editors and Affiliations

  1. Quality Assistance S.A., Donstiennes, Belgium

    Arnaud Delobel

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Giménez, E., Mancera-Arteu, M., Benavente, F., Sanz-Nebot, V. (2021). Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. In: Delobel, A. (eds) Mass Spectrometry of Glycoproteins. Methods in Molecular Biology, vol 2271. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1241-5_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1241-5_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1240-8

  • Online ISBN: 978-1-0716-1241-5

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