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Liquid chromatography-tandem mass spectrometry-based fragmentation analysis of glycopeptides

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Abstract

The use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MSn) for the glycoproteomic characterization of glycopeptides is a growing field of research. The N- and O-glycosylated peptides (N- and O-glycopeptides) analyzed typically originate from protease-digested glycoproteins where many of them are expected to be biomedically important. Examples of LC-MS2 and MS3 fragmentation strategies used to pursue glycan structure, peptide identity and attachment-site identification analyses of glycopeptides are described in this review. MS2 spectra, using the CID and HCD fragmentation techniques of a complex biantennary N-glycopeptide and a core 1 O-glycopeptide, representing two examples of commonly studied glycopeptide types, are presented. A few practical tips for accomplishing glycopeptide analysis using reversed-phase LC-MSn shotgun proteomics settings, together with references to the latest glycoproteomic studies, are presented.

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Acknowledgments

I would like to thank past and present members of the glycobiology group at clinical chemistry headed by Prof. Göran Larson. Financial support from the following foundations is gratefully acknowledged: Magnus Bergvall, Demensförbundet, WoM Lundgren and the Alzheimer foundation, Sweden.

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  1. Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden

    Jonas Nilsson

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Nilsson, J. Liquid chromatography-tandem mass spectrometry-based fragmentation analysis of glycopeptides. Glycoconj J 33, 261–272 (2016). https://doi.org/10.1007/s10719-016-9649-3

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