Abstract
Mucin glycans are associated with the function of mucin in maintaining mucosal homeostasis. Therefore, the glycomic analysis of mucins is crucial. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is one of the most suitable methods for the glycomic analysis of mucin O-glycans. In this chapter, we describe methods for analyzing permethylated O-glycan alditols released from mucins by MALDI-TOF MS and MALDI-TOF tandem mass spectrometry (MALDI-TOF MS/MS).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dekker J, Rossen JWA, Büller HA et al (2002) The MUC family: an obituary. Trends Biochem Sci 27:126–131
Corfield AP (2015) Mucins: a biologically relevant glycan barrier in mucosal protection. Biochim Biophys Acta 1850:236–252
Karasawa F, Shiota A, Goso Y et al (2012) Essential role of gastric gland mucin in preventing gastric cancer in mice. J Clin Invest 122:923–934
Kawakubo M, Komura H, Goso Y et al (2019) Analysis of A4gnt knockout mice reveals an essential role for gastric sulfomucins in preventing gastritis cystica profunda. J Histochem Cytochem 67:759–770
Morelle W, Michalski J-C (2007) Analysis of protein glycosylation by mass spectrometry. Nat Protoc 2:1585–1602
Goso Y, Sugaya T, Ishihara K et al (2017) Comparison of methods to release mucin-type O-glycans for glycomic analysis. Anal Chem 89:8870–8876
Anumula KR, Taylor PB (1992) A comprehensive procedure for preparation of partially methylated alditol acetates from glycoprotein carbohydrates. Anal Biochem 203:101–108
Goso Y, Ishihara K, Kurihara M et al (1999) Rat gastric mucins recognized by monoclonal antibodies RGM21 and HIK1083: isolation of mucin species characteristic of the surface and glandular mucosa. J Biochem 126:375–381
Domon B, Costello CE (1988) A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates. Glycoconj J 5:397–409
Acknowledgments
This work was supported by the Japan Society for the Promotion of Science KAKENHI grant 25460934.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Goso, Y., Kurihara, M. (2024). MALDI-TOF MS/MS Analysis of Permethylated O-Glycan Alditols Released from Mucins. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_17
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3670-1_17
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3669-5
Online ISBN: 978-1-0716-3670-1
eBook Packages: Springer Protocols