Abstract
The isolation and screening of bacteria and fungi for the production of surface-active compounds has been the basis for the majority of the biosurfactants discovered to date. Hence, a wide variety of well-established and relatively simple methods are available for screening, mostly focused on the detection of surface or interfacial activity of the culture supernatant. However, the success of any biodiscovery effort, specifically aiming to access novelty, relies directly on the characteristics being screened for and the uniqueness of the microorganisms being screened. Therefore, given that rather few novel biosurfactant structures have been discovered during the last decade, advanced strategies are now needed to widen access to novel chemistries and properties. In addition, more modern Omics technologies should be considered to the traditional culture-based approaches for biosurfactant discovery. This chapter summarizes the screening methods and strategies typically used for the discovery of biosurfactants and highlights some of the Omics-based approaches that have resulted in the discovery of unique biosurfactants. These studies illustrate the potentially enormous diversity that has yet to be unlocked and how we can begin to tap into these biological resources.
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Acknowledgements
The authors thank Sabrina Linden, Lisa-Marie Kirschen, and Phillip Venter for their contributions to the figures. The scientific activities of ST and SK were financially supported by the Ministry of Culture and Research within the framework of the NRW-Strategieprojekt BioSC (No. 313/323-400-002 13) and by Federal Ministry of Education and Research in the Project GlycoX (grant number 031B0866A); MT, NS, and AB were supported through grants (UID 87326 and 105876) by the National Research Foundation.
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Trindade, M., Sithole, N., Kubicki, S., Thies, S., Burger, A. (2021). Screening Strategies for Biosurfactant Discovery. In: Hausmann, R., Henkel, M. (eds) Biosurfactants for the Biobased Economy. Advances in Biochemical Engineering/Biotechnology, vol 181. Springer, Cham. https://doi.org/10.1007/10_2021_174
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