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Biosurfactant production by Pseudomonas aeruginosa DSVP20 isolated from petroleum hydrocarbon-contaminated soil and its physicochemical characterization

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Abstract

Among 348 microbial strains isolated from petroleum hydrocarbon-contaminated soil, five were selected for their ability to produce biosurfactant based on battery of screening assay including hemolytic activity, surface tension reduction, drop collapse assay, emulsification activity, and cell surface hydrophobicity studies. Of these, bacterial isolate DSVP20 was identified as Pseudomonas aeruginosa (NCBI GenBank accession no. GQ865644) based on biochemical characterization and the 16S rDNA analysis, and it was found to be a potential candidate for biosurfactant production. Maximum biosurfactant production recorded by P. aeruginosa DSVP20 was 6.7 g/l after 72 h at 150 rpm and at a temperature of 30 °C. Chromatographic analysis and high-performance liquid chromatography-mass spectrometry (HPLC-MS) revealed that it was a glycolipid in nature which was further confirmed by nuclear magnetic resonance (NMR) spectroscopy. Bioremediation studies using purified biosurfactant showed that P. aeruginosa DSVP20 has the ability to degrade eicosane (97 %), pristane (75 %), and fluoranthene (47 %) when studied at different time intervals for a total of 7 days. The results of this study showed that the P. aeruginosa DSVP20 and/or biosurfactant produced by this isolate have the potential role in bioremediation of petroleum hydrocarbon-contaminated soil.

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Acknowledgments

The authors are grateful to Deanship of Scientific Research and College of Food and Agriculture Science Research, King Saud University Riyadh for providing research support.

Conflict of interest

We comfirm that there is no conflict of interest upon publication of this paper.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent was not necessary because the study did not involve human participants.

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    Authors and Affiliations

    1. Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University Riyadh, Riyadh, 11451, PO Box 2460, Saudi Arabia

      Mohammad Javed Ansari, Ahmad Al-Ghamdi, Nuru Adgaba & Khalid Ali Khan

    2. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, India

      Deepak Sharma, Mohammad Javed Ansari & Vikas Pruthi

    3. Waili Foundation for Science, Literature and Trading, Queens, NY, 11418, USA

      Noori Al-Waili

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    1. Deepak Sharma
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    2. Mohammad Javed Ansari
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    Correspondence to Noori Al-Waili.

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    Responsible editor: Robert Duran

    Deepak Sharma and Mohammad Javed Ansari contributed equally to this work.

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    Sharma, D., Ansari, M.J., Al-Ghamdi, A. et al. Biosurfactant production by Pseudomonas aeruginosa DSVP20 isolated from petroleum hydrocarbon-contaminated soil and its physicochemical characterization. Environ Sci Pollut Res 22, 17636–17643 (2015). https://doi.org/10.1007/s11356-015-4937-1

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