Abstract
Haemolysis has been used as an initial selection criterion for the primary isolation of surfactant-producing bacteria. Only 37 of 492 strains of different origins had haemolytic activity. These 37 strains, together with 49 non-haemolytic ones chosen at random, were studied for surface activity. Only five strains, all of them haemolytic, tested positive. Haemolysis and biosurfactant-production are thus probably associated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bosh, M.T., Robert, M., Mercadé, M., Espuny, M.J., Parra, J.L. & Guinea, J. 1988 Surface active compounds on microbial cultures. Tensides, Surfactants, Detergents, 25, 208–211.
Cooper, D. G. & Zajic, J.F. 1980 Surface acting compounds from microorganisms. Advances in Applied Microbiology 26, 229–253.
Foght, J.M., Gutnick, D.L. & Westlake, D.W.S. 1989 Effect of emulsion on biodegradation of crude oil by pure and mixed cultures. Applied and Environmental Microbiology 55, 36–42.
Francy, D.S., Thomas, J.M., Raymond, R.L. & Ward, C.H. 1991 Emulsification of hydrocarbons by surface bacteria. Journal of Industrial Microbiology 8, 237–246.
Gerson, D.F. & Zajic, J.E. 1979 Microbial biosurfactants. Process Biochemistry 14, 20–24.
Ghurye, G.L., Vipulanandan, C. & Willson, R.C. 1994 A practical approach to biosurfactant production using nonaseptic fermentation of mixed cultures. Biotechnology and Bioengineering 44, 661–666.
Guerra-Santos, L., Kappeli, O. & Fiechter, A. 1984 Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source. Applied and Environmental Microbiology 48, 301–305.
Haferburg, D., Hommel, R., Claus, R. & Kleber, H.P. 1986 Extracellular microbial lipids as biosurfactants. Engineering Biotechnology 33, 53–59.
Marchesi, J.R., Russell, N.J., White, G.F. & House, W.A. 1991 Effects of surfactant adsorption and biodegradability in the distribution of bacteria between sediments and water in a fresh water microcosm. Applied and Environmental Microbiology 57, 2507–2513.
Passeri, A., Schmith, M., Lang, S. Wagner, F., Wray, V., Pormba, K. & Gunkel, W. 1988 Marine microorganisms: formation and effects of biosurfactants. In Eighth International Biotechnology Symposium, Abstract Book, Paris, 17–22 July p. 292.
Persson, A. & Molin, G. 1987 Capacity for biosurfactant production of environmental Pseudomonas and Vibrionaceae growing on carbohydrates. Applied Microbiology and Biotechnology 26, 439–442.
Posgate, J.R. 1969 Viable counts and viability. In Methods in Microbiology, Vol. 1 Norris, J.R. & Ribbons, D.W. (editors), pp. 611–615. London: Academic Press.
Ramsay, B., McCarthy, J., Guerra-Santos, L., Kappeli, O. & Fiechter, A. 1988 Biosurfactant production and diauxic growth of Rhodococcus aurantiacus when using n-alkanes as the carbon source. Canadian Journal of Microbiology 34, 1209–1212.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Carrillo, P.G., Mardaraz, C., Pitta-Alvarez, S.I. et al. Isolation and selection of biosurfactant-producing bacteria. World J Microbiol Biotechnol 12, 82–84 (1996). https://doi.org/10.1007/BF00327807
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00327807